Effect of PP1D-1, a synthetic antiplatelet compound, on rabbit platelets

Japanese Journal of Pharmacology
C H LiaoC M Teng

Abstract

The antiplatelet mechanism of a synthetic compound, 2-chloro-3-methoxycarbonylpropionamido-1,4-naphthoquinone (PP1D-1), was studied by employing washed rabbit platelets in vitro. PP1D-1 concentration-dependently inhibited thrombin (0.1 U/ml)-, platelet-activating factor (2 ng/ml)-, collagen (10 microg/ml)-, arachidonic acid (100 microM)- and U46619 (1 microM)-induced aggregation and ATP release in washed rabbit platelets. The IC50 values of PP1D-1 for aggregation induced by the above inducers are 17.9+/-1.7, 9.8+/-1.1, 3.9+/-0.4, 1.8+/-0.3 and 1.7+/-0.3 microM, respectively. PP1D-1 did not affect platelet thromboxane B2 or prostaglandin D2 formation induced by arachidonic acid, indicating that it did not affect cyclooxygenase and thromboxane synthase activities. PP1D-1 significantly inhibited the formation of inositol 1,4,5-trisphosphate caused by these five platelet stimulators. Moreover, PP1D-1 inhibited the increase in intracellular calcium concentration induced by these agents. On the contrary, PP1D-1 did not inhibit thapsigargin-elevated intracellular calcium concentration in indomethacin-pretreated platelets, indicating it did not influence the effect of thapsigargin. According to these data, PP1D-1 exerts antiplatelet ef...Continue Reading

References

Jul 15, 1992·European Journal of Biochemistry·B Brüne, V Ullrich
Jun 1, 1992·Stroke; a Journal of Cerebral Circulation·G W Albers
Apr 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·O ThastrupA P Dawson
May 31, 1990·The New England Journal of Medicine·M D TripJ Vreeken
Jan 1, 1989·Physiological Reviews·W Siess
Feb 1, 1986·Chest·E GentonE W Salzman
Sep 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·H IshiiP W Majerus
Jun 22, 1987·Biochimica Et Biophysica Acta·C M TengC H Ouyang
Dec 1, 1986·Journal of the American College of Cardiology·D G Sherman, R G Hart
Jul 1, 1995·Drugs·K Schrör
Jul 1, 1994·The American Journal of Physiology·J GeigerU Walter
Dec 16, 1993·Biochimica Et Biophysica Acta·Y HashimotoK Kurokawa
Sep 2, 1993·The New England Journal of Medicine·H V Anderson, J T Willerson

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Citations

Aug 7, 2002·The Journal of Pharmacy and Pharmacology·Chang-Hui LiaoChe-Ming Teng
Sep 12, 2007·International Journal of Immunogenetics·M HarishankarP R Narayanan
Aug 23, 2008·Archiv der Pharmazie·Jin-Cherng LienSheng-Chu Kuo

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