Effect of preoperative chemoradiotherapy on the immunological status of rectal cancer patients.

Journal of Radiation Research
Kazuaki YasuiYasuto Akiyama

Abstract

The aim of the study was to investigate the effect of chemo-radiation on the genetic and immunological status of rectal cancer patients who were treated with preoperative chemoradiotherapy (CRT). The expression of immune response-associated genes was compared between rectal cancer patients treated (n = 9) and not-treated (n = 10) with preoperative CRT using volcano plot analysis. Apoptosis and epithelial-to-mesenchymal transition (EMT) marker genes were analysed by quantitative PCR (qPCR). Other markers associated with the tumor microenvironment (TME), such as tumor-infiltrating lymphocytes (TIL) and immune checkpoint molecules, were investigated using immunohistochemistry (IHC). The clinical responses of preoperative CRT for 9 rectal cancer patients were all rated as stable disease, while the pathological tumor regression score (TRG) revealed 6 cases of grade2 and 3 cases of grade1. According to the genetic signature of colon cancers, treated tumors belonged to consensus molecular subtype (CMS)4, while not-treated tumors had signatures of CMS2 or 3. CRT-treated tumors showed significant upregulation of EMT-associated genes, such as CDH2, TGF-beta and FGF, and cancer stem cell-associated genes. Additionally, qPCR and IHC demons...Continue Reading

References

Dec 24, 2005·The New England Journal of Medicine·Franck PagèsJérôme Galon
Nov 20, 2008·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Jeffrey S WeberEvan Hersh
Jan 18, 2011·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Anna M DahlinRichard Palmqvist
Jul 13, 2011·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Vincenzo ValentiniPhilippe Lambin
Apr 25, 2012·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Rolf SauerClaus Rödel
Jun 5, 2012·The New England Journal of Medicine·Suzanne L TopalianMario Sznol
Jun 5, 2012·The New England Journal of Medicine·Julie R BrahmerJon M Wigginton
Jul 20, 2012·Nature·UNKNOWN Cancer Genome Atlas Network
Jun 4, 2013·The New England Journal of Medicine·Jedd D WolchokMario Sznol
Feb 26, 2014·Annals of Surgical Oncology·Eiji ShintoJunji Yamamoto
Feb 24, 2015·Nature Genetics·Claudio IsellaEnzo Medico
Oct 13, 2015·Nature Medicine·Justin GuinneySabine Tejpar
Jul 28, 2016·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Tadashi AshizawaYasuto Akiyama
Jan 5, 2017·Nature Reviews. Cancer·Rodrigo DienstmannJosep Tabernero
May 10, 2017·Journal of Thoracic Oncology : Official Publication of the International Association for the Study of Lung Cancer·Xiaomei GongCaicun Zhou
Jun 17, 2019·International Journal of Clinical Oncology·Yojiro HashiguchiUNKNOWN Japanese Society for Cancer of the Colon and Rectum

❮ Previous
Next ❯

Citations

Dec 12, 2020·Translational Research : the Journal of Laboratory and Clinical Medicine·Ebunoluwa E OtegbeyeMatthew A Ciorba
Apr 4, 2021·Cancers·Claudia CorròThibaud Koessler

❮ Previous
Next ❯

Methods Mentioned

BETA
exome sequencing
PCR
dissection

Software Mentioned

Torrent Variant Caller ( TVC )
Excel
QuantStudio
GeneSpring GX

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis