Effect of preservatives on Shiga toxigenic phages and Shiga toxin of Escherichia coli O157:H7

Journal of Food Protection
Tomás SubilsClaudia Balagué

Abstract

Toxin synthesis by Shiga toxin-producing Escherichia coli (STEC) appears to be coregulated through the induction of the integrated bacteriophages that encode the toxin genes. These phages might be the principal means for the dissemination and release of Shiga toxins. We evaluated the effect of three common food preservatives, potassium sorbate, sodium benzoate, and sodium propionate, on the propagation of the phages and Shiga toxins. We tested each preservative at four concentrations, 1, 1.25, 2.5, and 5 mg/ml, both on free phages and on lysogenic phages in bacteria. We also evaluated the expression of a lambdoid phage, which was exposed to increasing concentrations of preservatives, by measuring β-galactosidase activity from SPC105, a transductant strain. Furthermore, we tested the effect of the preservatives on cytotoxigenic activity of Shiga toxin on Vero cells. We detected an increase of the inhibitory effect of the phage lytic activity, both in lysogenic and free phages, as the preservative concentration increased. However, the inhibition was higher on the lysogenic phages release than on free phages. Sodium benzoate and potassium sorbate were about equal at inhibiting phages; they were more effective than sodium propionat...Continue Reading

References

Jan 1, 1989·Clinical Microbiology Reviews·M A Karmali
May 1, 1989·Microbial Pathogenesis·A D O'BrienR K Holmes
Aug 1, 1989·Journal of General Microbiology·P J RietraB Rowe
Dec 1, 1982·The Journal of Infectious Diseases·A D O'BrienS B Formal
Sep 1, 1980·Journal of Clinical Microbiology·M K Gentry, J M Dalrymple
May 5, 1993·JAMA : the Journal of the American Medical Association·R E BesserP M Griffin
Apr 1, 1997·Pediatric Nephrology : Journal of the International Pediatric Nephrology Association·F D SpizzirriO R Amoreo
Mar 27, 1999·Journal of Bacteriology·A MatsushiroT Honda
Oct 26, 2001·Journal of Molecular Evolution·J Kuhn, A Campbell
Oct 9, 2003·Emerging Infectious Diseases·Marta RivasSergio Sosa Estani
Feb 3, 2004·American Journal of Infection Control·Judith Morrison, UNKNOWN Health Canada, Nosocomial and Occupational Infections Section
Feb 25, 2005·Antimicrobial Agents and Chemotherapy·Sylvia HeroldHerbert Schmidt
Mar 31, 2005·Current Opinion in Genetics & Development·Ian B DoddJ Barry Egan
Apr 22, 2005·Public Health Reports·Richard L Vogt, Laura Dippold
Oct 2, 2008·Emerging Infectious Diseases·Juliana GrantRobert T Rolfs

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Citations

Jan 28, 2014·Applied and Environmental Microbiology·Anna Allué-GuardiaMaite Muniesa
Jul 20, 2019·Expert Review of Anti-infective Therapy·Ewa Jończyk-MatysiakAndrzej Górski
Jul 25, 2019·Pathogens·Steven BatinovicAshley E Franks

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