Effect of preserving condition of porcine ovaries on the development of in vitro matured oocytes

Reproduction in Domestic Animals = Zuchthygiene
Y-A LinM-C Chen

Abstract

The effect of preservation condition of ovaries on the in vitro maturation of the porcine oocytes was studied. Cumulus-oocyte complexes (COCs) were obtained from the ovaries preserved in Dulbecco's phosphate buffered saline (PBS) solution at various temperatures for different time intervals, and cultured in M199 maturation medium. Matured oocytes were obtained from the ovaries preserved in PBS for 8 h and electrically activated. The activated oocytes were then cultured in NCSU23 embryo culture medium for 16 h to observe activation or 144 h to observe embryo development. It was found that the preservation temperature affected the maturation of porcine oocytes greatly. The effect was described as a compromise of the suppressions of autolysis at physiological temperature and frostbite because of low temperature. A preservation temperature of approximately 25°C showed the maximum maturation rate for a preservation time of 8 h. Preservation temperature also affected the activation and embryo development of porcine oocytes greatly, following a trend similar to the effect of preservation temperature on the maturation. Based on maturation rate, activation rate and cleavage rate, a preservation temperature of approximately 25°C would be...Continue Reading

References

Apr 1, 1991·Molecular Reproduction and Development·R S PratherM S Peters
Nov 26, 1997·Journal of Reproduction and Fertility·W H WangB N Day
Aug 1, 1998·Biology of Reproduction·Z MachátyR S Prather
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Jul 11, 2001·Small Ruminant Research : the Journal of the International Goat Association·E R. AndradeJ R. Figueiredo
Feb 1, 2003·Theriogenology·Lucyna Katska-KsiazkiewiczMarek Pieńkowski
Feb 5, 2003·Theriogenology·Yindee KitiyanantKanok Pavasuthipaisit
Dec 14, 2005·Animal Reproduction Science·Muzaffer TaşSerhat Pabuccuoğlu

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