PMID: 9546058Apr 18, 1998Paper

Effect of pressure on the catalytic activity of subtilisin Carlsberg suspended in compressed gases

Biochimica Et Biophysica Acta
N FontesSusana Barreiros

Abstract

We studied the effect of pressure up to 300 bar on the catalytic efficiency of subtilisin Carlsberg suspended in compressed propane, near-critical ethane, near-critical carbon dioxide and tert-amyl alcohol, at constant temperature and fixed enzyme hydration. Increasing pressure lowered the catalytic efficiency of the enzyme in all the solvents, resulting in positive activation volumes, delta V#. The delta V# values in compressed propane and in tert-amyl alcohol were similar and larger in magnitude than the value reported in the literature for the same reaction in an aqueous buffer, although within the range of typical delta V# values in aqueous media. In the near-critical fluids, the delta V# were much larger, e.g., an increase in pressure of only 200 bar causing a sixfold decrease in the catalytic efficiency of subtilisin in carbon dioxide. These data should reflect the proximity of ethane and carbon dioxide to the critical point, and the resulting condensation of solvent molecules about the solutes, yielding negative solute partial molar volumes.

References

Feb 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·R AffleckJ S Dordick
Apr 1, 1992·Enzyme and Microbial Technology·S KamatA J Russell
Mar 20, 1988·Journal of Molecular Biology·C E Kundrot, F M Richards
Jan 1, 1981·Advances in Protein Chemistry·E Morild
Apr 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·S V KamatA J Russell
Jan 1, 1996·Proteins·V V MozhaevC Balny
Feb 5, 1992·Biotechnology and Bioengineering·A MartyJ S Condoret
Sep 5, 1993·Biotechnology and Bioengineering·J Kim, J S Dordick
May 5, 1996·Biotechnology and Bioengineering·T C de SampaioS Barreiros
Oct 20, 1996·Biotechnology and Bioengineering·V V MozhaevC Balny

❮ Previous
Next ❯

Citations

Feb 19, 2000·Current Opinion in Chemical Biology·P J Halling
Sep 6, 2001·Biotechnology and Bioengineering·J A Laszlo, D L Compton
Jul 30, 2010·Chemistry : a European Journal·María J HernáizJosé V Sinisterra
Sep 15, 1999·Biotechnology and Bioengineering·M SarkariC S Chen
Feb 13, 2008·The Journal of Physical Chemistry. B·Nuno M Micaêlo, Claudio M Soares

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.