Effect of presumptive tests reagents on human blood confirmatory tests and DNA analysis using real time polymerase chain reaction

Forensic Science International : Synergy
Juliana Piva de AlmeidaCristina Bonorino

Abstract

Bloodstains often constitute the major physical evidence in crime investigation. Diluted blood invisible to the naked eye can be detected through presumptive tests however such tests can damage samples and prevent further processing such as DNA analysis. In this study, we compared the effects of luminol (prepared according to Weber [15]), Luminol 16(®), Bluestar(®) and benzidine for inhibition in the human antiglobulin test and the human hemoglobin immunochromatographic test and on the total human DNA concentration up to 120 days after sample treatment. Treatment with both luminol solutions and Bluestar(®) still allowed positive results for the immunologic tests, indicating non-interference with human blood confirmatory tests. However, samples treated with benzidine could not be further analyzed by serological tests. Also, DNA quantification showed that 48h after benzidine treatment, but not luminol or Bluestar solution application, sample DNA was degraded. Luminol 16(®) caused DNA degradation already at 30 days post-application. At 120 days post treatment, all samples treated with any of the agents but not untreated samples had DNA degradation.

References

May 16, 2000·Forensic Science International : Synergy·A CarracedoM Wilson
Jan 11, 2005·Forensic Science International : Synergy·G TringaliA M Roccazzello
Nov 21, 2008·Journal of Forensic Sciences·Karolyn L TontarskiAmy L Michaud
Jul 21, 2010·Forensic Science International : Synergy·Juliana Piva de AlmeidaCristina Bonorino
Jan 21, 2014·Forensic Science, Medicine, and Pathology·Marielle VennemannShanan S Tobe

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Citations

Jan 10, 2016·Journal of Forensic and Legal Medicine·Ivana HorjanGordan Mrsic
May 11, 2016·Science & Justice : Journal of the Forensic Science Society·Wee Chuen LeeAhmad Fahmi Lim Abdullah
Mar 5, 2016·International Journal of Legal Medicine·V Sterzik, M Bohnert
Oct 5, 2012·Journal of Forensic Sciences·Sarah J SeasholsAshley Rief
Apr 13, 2015·Forensic Science International. Genetics·Gillian E DonachieNicholas D Tribble
May 14, 2016·Forensic Science International : Synergy·A G Skalleberg, M M Bouzga
Jul 21, 2010·Forensic Science International : Synergy·Juliana Piva de AlmeidaCristina Bonorino
Jan 28, 2018·International Journal of Legal Medicine·V BarreraB Fliss
Jun 1, 2019·Journal of Forensic Sciences·Julie ButlerKirsty Wright

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