Effect of pretest storage conditions of extracted teeth on their dentin bond strengths

The Journal of Prosthetic Dentistry
E H MobarakHana Jamjoom

Abstract

It is becoming increasingly difficult to obtain sufficient freshly extracted human teeth to utilize in in vitro studies, particularly for bonding investigations. The purpose of this study was to investigate the effect of different storage conditions for human extracted teeth prior to their use in microtensile bond strength (microTBS) testing. Thirty incisors were divided into groups according to their storage time and condition. The first group consisted of freshly extracted teeth. Teeth of the second group were stored in chloramine-T at 4 degrees C for 2 years +/-1 month. The last group was stored dry for up to 2 years +/-2 months, after which teeth were rehydrated in distilled water for 2 weeks before use. All teeth were disinfected using 0.5% chloramine-T. Dentin surfaces were exposed and then bonded using Scotchbond Multi-Purpose or Clearfil SE Bond to composite resin foundations (Esthet-X). Following 24-hour storage, bonded teeth (n=30) were sectioned into beams with a cross-section of 0.9 +/-0.1 mm(2) and subjected to microTBS testing using a microtensile testing machine (Micro Tensile Tester). Data were calculated and statistically analyzed using Kruskal-Wallis and Mann-Whitney tests (alpha=.05). Failure modes were deter...Continue Reading

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Citations

Nov 24, 2016·Microscopy Research and Technique·Muhammet Kerim Ayar, Fatih Erdemir
Apr 29, 2020·Biomaterial Investigations in Dentistry·Hamid KermanshahMutlu Özcan
Jan 30, 2021·European Journal of Dentistry·Gabriela Simões TeixeiraAlexandre Henrique Susin
Jun 19, 2014·The Journal of Contemporary Dental Practice·Cynthia Soares de AzevedoAdriana Bona Matos
Apr 26, 2018·Dental Materials : Official Publication of the Academy of Dental Materials·Xuekun LuPhilip J Withers

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