Effect of primary degradation-reaction products from Ammonia Fiber Expansion (AFEX)-treated corn stover on the growth and fermentation of Escherichia coli KO11

Bioresource Technology
Ming W Lau, Bruce Dale

Abstract

The primary degradation-reaction products (DRP) identified in Ammonia Fiber Expansion (AFEX)-pretreated corn stover are acetate, lactate, 4-hydroxybenzaldehyde (4HBD) and acetamide. The effects of these products at a broad concentration range were tested on Escherichia coli KO11, a strain engineered for cellulosic ethanol production. Fermentations using glucose or xylose as the sole carbohydrate source and a sugar mixture of glucose and xylose were conducted to determine how these products and sugar selection affected fermentation performance. Co-fermentation of the sugar mixture exhibited the lowest overall ethanol productivity compared to single-sugar fermentations and was more susceptible to inhibition. Metabolic ethanol yield increased with the increasing initial concentration of acetate. Although these degradation-reaction products (with exception of acetamide) are generally perceived to be inhibitory, organic acids and 4-hydroxybenzaldehyde at low levels stimulated fermentation. Adaptation of cells to these products prior to fermentation increased overall fermentation rate.

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Jan 24, 2009·Proceedings of the National Academy of Sciences of the United States of America·Ming W Lau, Bruce E Dale

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Citations

May 24, 2011·Current Opinion in Biotechnology·Claudia C GeddesLonnie O Ingram
Feb 25, 2011·Bioresource Technology·DeLu Tyler YinRomas J Kazlauskas
Feb 3, 2015·Biotechnology for Biofuels·Xiaoyu TangVenkatesh Balan
Dec 20, 2011·Bioresource Technology·Michaela KolbDoris Schieder
Dec 7, 2010·Bioresource Technology·Dawei YuanSasidhar Varanasi
Feb 2, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Daehwan Kim

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