PMID: 9653629Jul 8, 1998Paper

Effect of process conditions on recovery of protein activity after freezing and freeze-drying

European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V
S Jiang, Steven L Nail

Abstract

The objective of this research was to gain a better understanding of the degree to which recovery of activity of model proteins after freeze-drying can be maximized by manipulation of freeze-dry process conditions in the absence of protective solutes. Catalase, beta-galactosidase and lactate dehydrogenase (LDH) were used as model proteins. All of the three proteins exhibited a concentration-dependent loss of activity after freezing, with significantly higher recovery at higher concentration. The freezing method and the type of buffer were also important, with sodium phosphate buffer and freezing by immersion of vials in liquid nitrogen associated with the lowest recovery of activity. Differential scanning calorimetry was predictive of the onset of collapse during freeze-drying only for beta-galactosidase. For the other proteins, either no Tg' transition was observed, or the apparent glass transition did not correlate with the microscopically-observed collapse temperature. The time course of activity loss for beta-galactosidase and LDH was compared during freeze-drying under conditions which produced collapse of the dried matrix and conditions which produced retention of microstructure in the dried solid. Recovery of activity de...Continue Reading

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Citations

Jun 1, 2011·Applied Microbiology and Biotechnology·Baokun LiWei Chen
May 7, 2013·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·R Geidobler, G Winter
Jun 12, 2013·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Arnab GangulySherry G Kim
Jun 12, 2013·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Julia Christina KasperWolfgang Friess
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Jul 3, 2003·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Hartwig SteckelKlaus Witthohn
Jul 31, 2009·Journal of Biomechanical Engineering·Stéphanie PassotFernanda Fonseca
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Dec 10, 1999·Journal of Pharmaceutical Sciences·J M SarciauxS L Nail
Feb 13, 2013·Journal of Pharmaceutical Sciences·Maria A MillerJennifer A Maynard
Jan 26, 2007·Journal of Pharmaceutical Sciences·Ahmad M Abdul-FattahMichael J Pikal
Aug 6, 2004·Journal of Pharmaceutical Sciences·Ketan AminBarbara Wang
Jun 23, 2009·Journal of Pharmaceutical Sciences·J Jeff SchwegmanSteven L Nail
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Jun 6, 2009·Journal of Pharmaceutical Sciences·Teresa Cochran, Steven L Nail
Apr 7, 2004·Journal of Pharmaceutical Sciences·D Q WangS L Nail
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Mar 24, 2011·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Julia Christina Kasper, Wolfgang Friess
Feb 15, 2011·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·N Perez-MoralR Parker
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Apr 6, 2016·Journal of Pharmaceutical Sciences·Lavanya K IyerElizabeth M Topp

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