Effect of products of PLA2 catalyzed hydrolysis of DLPC on motion of rising bubbles

Colloids and Surfaces. B, Biointerfaces
M KrzanEwa Rogalska

Abstract

Local velocities of rising bubbles decrease with the increasing concentration in solution of surface-active, water-soluble species. Therefore, it is possible to use this phenomenon to monitor products of enzymatic reactions, which meet such criteria. In this study, hydrolysis of 1,2-dilauroyl-sn-glycero-3-phosphatidylcholine (DLPC) catalyzed by calcium-dependent phospholipase A2 (PLA2) (EC3.1.1.4) from porcine pancreas was used as model reaction. The products of this reaction are lauric acid (LA) and 1-lauroyl-2-hydroxy-sn-glycero-3-phosphatidylcholine (Lyso-PC). DLPC was dispersed in a chloroform/methanol mixture that was spread on a free PLA2 solution surface. Air bubbles were then formed at a capillary orifice and the local velocity of rising bubbles as a function of the distance from the capillary tip was monitored. Local velocity profiles were compared with profiles recorded for solutions of pure enzymatic reaction products and their mixtures. Our experiments showed that the product, which had a dominating effect on bubble motion retardation, was lyso-phosphatidylcholine. This can be explained by differences in the kinetics of lauric acid and lyso-phosphatidylcholine transfer from the spread layer to the solution.

References

Jan 1, 1976·Annual Review of Biophysics and Bioengineering·R Verger
Feb 1, 1973·Chemistry and Physics of Lipids·R Verger, G H De Haas
Mar 23, 2000·Journal of Colloid and Interface Science·Y Liao, J B McLaughlin
Nov 18, 2000·Biochimica Et Biophysica Acta·D A Six, E A Dennis
Nov 16, 2002·Angewandte Chemie·Patricia Moraille, Antonella Badia
Apr 20, 2005·Langmuir : the ACS Journal of Surfaces and Colloids·Alissa J ProsserKlaus Lunkenheimer
Dec 28, 2010·Progress in Lipid Research·Makoto MurakamiKei Yamamoto

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Citations

Feb 13, 2020·Langmuir : the ACS Journal of Surfaces and Colloids·Marco SchnurbusBjörn Braunschweig

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