Effect of progressive inoculation of fauna-free sheep with holotrich protozoa and total-fauna on rumen fermentation, microbial diversity and methane emissions

FEMS Microbiology Ecology
Alejandro BelancheCharles J Newbold

Abstract

Rumen methanogenesis represents an energy waste for the ruminant and an important source of greenhouse gas; thus, integrated studies are needed to fully understand this process. Eight fauna-free sheep were used to investigate the effect of successive inoculation with holotrich protozoa then with total fauna on rumen methanogenesis. Holotrichs inoculation neither altered rumen fermentation rate nor diet digestibility, but increased concentrations of acetate (+15%), butyrate (+57%), anaerobic fungi (+0.82 log), methanogens (+0.41 log) and methanogenesis (+54%). Further inoculation with total fauna increased rumen concentrations of protozoa (+1.0 log), bacteria (+0.29 log), anaerobic fungi (+0.78 log), VFA (+8%), ammonia and fibre digestibility (+17%) without affecting levels of methanogens or methanogenesis. Rumen methanogens population was fairly stable in terms of structure and diversity, while the bacterial community was highly affected by the treatments. Inoculation with holotrich protozoa increased bacterial diversity. Further inoculation with total fauna lowered bacterial diversity but increased concentrations of certain propionate and lactate-producing bacteria, suggesting that alternative H2 sinks could be relevant. This ...Continue Reading

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Citations

Dec 5, 2015·Frontiers in Microbiology·Charles J NewboldNeil R McEwan
Oct 7, 2015·Journal of the Science of Food and Agriculture·Alejandro BelancheC Jamie Newbold
Nov 4, 2015·Frontiers in Microbiology·David R Yáñez-RuizCharles J Newbold
Aug 25, 2018·Animal : an International Journal of Animal Bioscience·I Mizrahi, E Jami
Sep 14, 2019·Environmental Microbiology·Alejandro BelancheCharles J Newbold
Feb 7, 2020·Animal : an International Journal of Animal Bioscience·C J Newbold, E Ramos-Morales
Oct 28, 2020·Environmental Microbiology Reports·Ronnie Solomon, Elie Jami
May 8, 2019·Animal : an International Journal of Animal Bioscience·R J GruningerT A McAllister
May 14, 2021·Nature Reviews. Microbiology·Itzhak MizrahiSarah Moraïs
Jun 3, 2021·Animals : an Open Access Journal From MDPI·Brooke A ClemmonsPhillip R Myer
Aug 12, 2021·Scientific Reports·Saheed A SalamiAlessandro Priolo

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Methods Mentioned

BETA
PCR

Software Mentioned

GenStat
TRFLP
PRIMER

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