Effect of prophylactic transfusion of stored RBCs on oxygen reserve in response to acute isovolemic hemorrhage in a rodent model

Transfusion
M S d'AlmeidaIan Chin-Yee

Abstract

The storage of RBCs results in a time-related decline in 2,3 DPG that may reduce the ability to unload oxygen (O(2)) to tissue. The objective of this study was to compare the effect that transfusion of stored 2,3 DPG-depleted rat blood (7 days in CPDA-1) had on the O(2) reserve in conscious rats, with that of the transfusion of fresh blood (<2-hour storage). Anemic rats (Hb, 80 g/L) received either fresh packed RBCs or stored RBCs to raise Hb levels to 140 g per L. They then underwent isovolemic hemorrhage mimicking surgical blood loss to the point of O(2) supply dependency (OSD). Critical O(2) delivery (DO(2)crit), Hb concentration, and O(2) extraction at OSD were measured in a metabolic chamber. After transfusion, RBC DPG decreased by 50 percent in the stored-blood group, and the p50 value decreased by 5 mmHg (32.1 +/- 2.5 mmHg vs. 37.5 +/- 3.0). DO(2)crit was similar in the two groups (fresh blood: 2.79 +/- 0.44 mL/min x g(-1); stored blood, 2.99 +/- 0.76 mL/min x g(-1)). The critical Hb concentration at DO(2)crit was higher in the stored-blood group (44 +/- 4 g/L) than in the fresh-blood group (38 +/- 5 g/L); the cardiac index and O(2) extraction ratio in the two groups were not different. Under conditions of severe normovo...Continue Reading

References

Dec 1, 1975·Scandinavian Journal of Clinical and Laboratory Investigation·G Arturson, M Westman
Feb 1, 1977·Journal of Applied Physiology: Respiratory, Environmental and Exercise Physiology·S M Cain
Mar 1, 1991·Journal of Applied Physiology·M C HoganP D Wagner
Jan 1, 1986·Canadian Journal of Physiology and Pharmacology·C K Chapler, S M Cain
May 1, 1988·Journal of Applied Physiology·R W Samsel, P T Schumacker
Jun 1, 1973·Journal of Applied Physiology·P W RandW H Austin
Nov 1, 1973·The Journal of Clinical Investigation·R D WoodsonJ C Detter
Dec 1, 1971·Journal of Applied Physiology·C R Valeri, F B Collins
Jan 1, 1971·Annals of Internal Medicine·F A OskiL D Miller
Oct 1, 1993·Journal of Applied Physiology·J C Stein, M L Ellsworth
Nov 1, 1996·World Journal of Surgery·U Kreimeier, K Messmer

Citations

Jan 16, 2004·Critical Care Medicine·Juliet HoIan Chin-Yee
May 14, 2005·Critical Care Medicine·J R Hess, Richard P Dutton
Aug 13, 2005·Critical Care Medicine·Bruce D Spiess
Sep 23, 2011·Critical Care : the Official Journal of the Critical Care Forum·Elizabeth C ParsonsNHLBI ARDS Network
Feb 3, 2012·Critical Care : the Official Journal of the Critical Care Forum·John-Paul TungYoke L Fung
Jul 24, 2012·Seminars in Perinatology·Allan Doctor, Phil Spinella
Jun 19, 2010·Transfusion and Apheresis Science : Official Journal of the World Apheresis Association : Official Journal of the European Society for Haemapheresis·J R Hess
Jun 29, 2010·Transfusion and Apheresis Science : Official Journal of the World Apheresis Association : Official Journal of the European Society for Haemapheresis·Arif SomaniRobert P Hebbel
Nov 17, 2009·Journal of Proteomics·J R Hess
Jan 21, 2009·Vox Sanguinis·A B Zimrin, J R Hess
Jan 11, 2012·Transfusion·J R Hess, Biomedical Excellence for Safer Transfusion (BEST) Collaborative
Jan 23, 2014·Vox Sanguinis·J R Hess
Nov 28, 2014·Journal of Veterinary Emergency and Critical Care·Rafael ObradorBernie Hansen
Sep 16, 2004·Journal of Cardiothoracic and Vascular Anesthesia·Bruce D Spiess
Apr 9, 2016·Journal of Liposome Research·Luciana da Silveira CavalcanteJelena L Holovati
Jul 19, 2016·Indian Journal of Hematology & Blood Transfusion : an Official Journal of Indian Society of Hematology and Blood Transfusion·R SoumyaR Vani
May 30, 2009·Journal of the American College of Cardiology·Brendan J DoyleDavid R Holmes
Jan 30, 2007·Hematology/oncology Clinics of North America·Bruce D Spiess
Apr 24, 2007·American Journal of Physiology. Heart and Circulatory Physiology·Pedro Cabrales

Related Concepts

Anemia
Metazoa
Anoxemia
Blood Preservation
Hemorrhage
Dioxygen
Blood Loss, Surgical
Rats, Holtzman
Red Blood Cell Transfusion
Rats, Laboratory

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