Effect of prostaglandins against alloxan-induced cytotoxicity to insulin secreting insulinoma RIN cells in vitro

Prostaglandins, Leukotrienes, and Essential Fatty Acids
M M Sailaja Devi, U N Das

Abstract

In the present study, we studied the effect of various prostaglandins (PGs) on alloxan-induced cytotoxicity to rat insulinoma (RIN) cells. Of all the PGs tested, PGE(1), PGE(2), PGI(2), PGF(1 alpha), and PGF(3 alpha) protected RIN cells from alloxan-induced cytotoxicity (P<0.05 compared to alloxan), whereas thromboxane B(2) and 6-keto-PGF(1 alpha) were not effective. PGE(1) induces a statistically significant increase in the activities of superoxide dismutase and glutathione peroxidase and decrease in lipid peroxides in alloxan-treated RIN cells (P<0.001). PGE(1) restored nitric oxide/lipid peroxide ratio to normalcy, suggesting that PGE(1) suppresses oxidant stress induced by alloxan in RIN cells in vitro. Furthermore, PGE(1) prevented DNA damage and apoptosis induced by alloxan. These results indicate that PGE(1) prevents alloxan-induced cytotoxicity to RIN cells in vitro.

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Nov 22, 2005·Prostaglandins, Leukotrienes, and Essential Fatty Acids·M M Sailaja Devi, U N Das

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Citations

Jun 1, 2005·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Abir T El-AlfyAmal J Fatani
Sep 1, 2007·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Alexandros PapadimitriouShanta J Persaud
Oct 15, 2013·Archives of Medical Research·Jun-hui ShenUndurti N Das
Oct 22, 2011·Prostaglandins, Leukotrienes, and Essential Fatty Acids·Undurti N Das
Nov 22, 2005·Prostaglandins, Leukotrienes, and Essential Fatty Acids·M M Sailaja Devi, U N Das
Aug 22, 2017·Frontiers in Endocrinology·Undurti N Das
Sep 28, 2018·Current Medicinal Chemistry·Curtis W PazderkaTristan Rawling

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