Effect of proteins on the assessment of surfactant cytotoxicity by an in vitro test: Possible correlations with in vivo data

Toxicology in Vitro : an International Journal Published in Association with BIBRA
J BenoitJ Wepierre

Abstract

Four surfactants were studied for their cytotoxic effect on human fibroblasts in culture. The surfactants were sodium dodecyl sulphate (SDS; anionic), cetyltrimethylammonium bromide (CTAB; cationic) and two nonionic compounds, octylphenoxy polyethoxyethanol (Triton X100) and polyoxyethylene (20) sorbitan monooleate (Tween 80). For each surfactant, LD(100) values were determined with varying amounts of protein-foetal calf serum (FCS) or bovine serum albumin (BSA)-in the culture medium, to evaluate the influence of protein concentration on surfactant cytotoxicity. FCS, like BSA, acted as a detoxifying agent, increasing the LD(100) values especially for SDS and Tween 80. Dialysis experiments enabled this general decrease in cytotoxicity to be correlated with a binding process. The binding of surfactants to the proteins of the culture medium could also be estimated by calculation, using the LD(100) values, and the values obtained were found to be similar to those determined by the dialysis assays. Comparison of these in vitro cytotoxicity results with those obtained by an in vivo procedure (the Draize test) showed that the protein in the culture medium was essential for the establishment of good correlations. Thus, this in vitro te...Continue Reading

References

Oct 1, 1976·Food and Cosmetics Toxicology·T F Ferguson, C Prottey
Feb 1, 1985·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·E Bosshard
Feb 1, 1985·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·C A ReinhardtG Zbinden
Feb 1, 1985·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·M C Scaife
Feb 1, 1985·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·C ShopsisD M Stark
Nov 1, 1971·The Journal of Pharmacy and Pharmacology·S M Metcalfe
Jun 1, 1971·Toxicology and Applied Pharmacology·C S Weil, R A Scala
Jan 1, 1983·Annals of the New York Academy of Sciences·B Ekwall
Oct 1, 1984·Cell Biology and Toxicology·E Borenfreund, O Borrero
Jan 1, 1980·Toxicology·B Ekwall
Jan 1, 1981·Toxicology·A P StammatiF Zucco
Oct 1, 1982·International Journal of Cosmetic Science·M C Scaife

❮ Previous
Next ❯

Citations

Jan 1, 1990·Toxicology in Vitro : an International Journal Published in Association with BIBRA·V Bianchi, E Fortunati
Jan 1, 1990·Toxicology in Vitro : an International Journal Published in Association with BIBRA·E CombrierM Adolphe
Jan 1, 1991·Toxicology in Vitro : an International Journal Published in Association with BIBRA·C CohenR Roguet
Mar 1, 1992·Toxicology in Vitro : an International Journal Published in Association with BIBRA·M CornelisJ Wepierre
Jul 1, 1993·Toxicology in Vitro : an International Journal Published in Association with BIBRA·R W LewisI Kimber
Jan 1, 1993·Toxicology in Vitro : an International Journal Published in Association with BIBRA·M Gülden
Aug 1, 1994·Toxicology in Vitro : an International Journal Published in Association with BIBRA·H H BohetsP J Dierickx
Aug 1, 1994·Toxicology in Vitro : an International Journal Published in Association with BIBRA·R K WardR H Clothier
May 22, 2002·Toxicology in Vitro : an International Journal Published in Association with BIBRA·H SeibertM Gülden
Oct 1, 1997·Toxicology in Vitro : an International Journal Published in Association with BIBRA·M Gülden, H Seibert
Apr 1, 1998·Toxicology in Vitro : an International Journal Published in Association with BIBRA·R K WardR H Clothier
Jan 24, 2009·European Journal of Orthodontics·Pertti PirttiniemiHans U Luder
Jul 8, 2000·Annual Review of Public Health·J A PatzJ Last
Mar 9, 2006·Alternatives to Laboratory Animals : ATLA·Sandra CoeckeAndrew Worth
Nov 1, 2012·Human & Experimental Toxicology·Aranya ManosroiJ Manosroi

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Toxicology in Vitro : an International Journal Published in Association with BIBRA
J M Frazier
Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association
C A ReinhardtG Zbinden
Toxicology in Vitro : an International Journal Published in Association with BIBRA
H H BohetsP J Dierickx
Toxicology in Vitro : an International Journal Published in Association with BIBRA
C FlowerD Anderson
Xenobiotica; the Fate of Foreign Compounds in Biological Systems
A B Fischer
© 2021 Meta ULC. All rights reserved