Effect of protocatechuic acid on insulin responsiveness and inflammation in visceral adipose tissue from obese individuals: possible role for PTP1B

International Journal of Obesity : Journal of the International Association for the Study of Obesity
Paulina OrmazabalRoberta Masella

Abstract

The occurrence of chronic inflammation in visceral adipose tissue (VAT) in obese subjects precipitates the development of insulin resistance and type 2 diabetes (T2D). Anthocyanins and their main metabolite protocatechuic acid (PCA) have been demonstrated to stimulate insulin signaling in human adipocytes. The aim of this study was to investigate whether PCA is able to modulate insulin responsiveness and inflammation in VAT from obese (OB) and normal weight (NW) subjects. VATs obtained from NW and OB subjects were incubated or not (control) with 100 μM PCA for 24 h. After incubation, tissues untreated and treated with PCA were acutely stimulated with insulin (20 nM, 20 min). PTP1B, p65 NF-κB, phospho-p65 NF-κB, IRS-1, IRβ, Akt, GLUT4 as well as basal and insulin-stimulated Tyr-IRS-1 and Ser-Akt phosphorylations were assessed by Western blotting in NW- and OB-VAT. Samples were assessed for PTP1B activity and adipocytokine secretion. PCA restored insulin-induced phosphorylation in OB-VAT by increasing phospho-Tyr-IRS-1 and phospho-Ser-Akt after insulin stimulation as observed in NW-VAT (p < 0.05). PTP1B activity was lower in OB-VAT treated with PCA with respect to untreated (p < 0.05). Compared to non-treated tissues, PCA reduced...Continue Reading

References

Feb 27, 2001·Molecular and Cellular Endocrinology·X Y WangJ E Bleasdale
May 2, 2002·Cell·Sankar Ghosh, Michael Karin
Dec 6, 2003·Science·Morris F White
Jul 2, 2004·The British Journal of Nutrition·Colin D KayJian Wang
Feb 26, 2005·Arteriosclerosis, Thrombosis, and Vascular Biology·Menno P J de WintherMarten H Hofker
Oct 28, 2005·The Journal of Nutrition·Colin D KayBruce J Holub
Aug 22, 2007·The Journal of Nutrition·Paola VitaglioneVincenzo Fogliano
May 6, 2009·Molecular Nutrition & Food Research·Colin D KayAedin Cassidy
Sep 3, 2009·World Journal of Gastroenterology : WJG·Shinji KawahitoShuzo Oshita
May 19, 2010·International Journal of Molecular Sciences·Kati HanhinevaKaisa Poutanen
Aug 19, 2010·Oxidative Medicine and Cellular Longevity·Kanti Bhooshan Pandey, Syed Ibrahim Rizvi
Oct 20, 2010·The British Journal of Nutrition·Gary Williamson, Michael N Clifford
Nov 8, 2011·Methods in Molecular Biology·Kirstin A CarswellSusan K Fried
Mar 24, 2012·Journal of Agricultural and Food Chemistry·Maria HidalgoSonia de Pascual-Teresa
Apr 28, 2012·International Journal of Food Sciences and Nutrition·Zahra BahadoranParvin Mirmiran
Jun 2, 2012·Pharmacognosy Reviews·Pathirage Kamal Perera, Yunman Li
Aug 15, 2012·Immunological Reviews·Amy R JohnsonLiza Makowski
Nov 22, 2012·Population Health Metrics·Gretchen A StevensUNKNOWN Global Burden of Metabolic Risk Factors of Chronic Diseases Collaborating Group (Body Mass Index)
Dec 26, 2012·Clinica Chimica Acta; International Journal of Clinical Chemistry·Amita YadavAnil Yadav
Feb 5, 2013·Vitamins and Hormones·Hyeongjin Cho
Apr 23, 2013·The American Journal of Clinical Nutrition·Charles CzankColin D Kay
Mar 8, 2014·British Journal of Pharmacology·R M de FerrarsC D Kay
May 7, 2015·Molecular Nutrition & Food Research·Beatrice ScazzocchioRoberta Masella
Jul 30, 2015·European Journal of Immunology·Felix M WensveenBojan Polić

❮ Previous
Next ❯

Citations

Aug 23, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Hélder OliveiraIva Fernandes
May 20, 2020·Diabetes, Metabolic Syndrome and Obesity : Targets and Therapy·Jia FengYi Ma
Jun 7, 2020·Journal of Physiology and Biochemistry·Francisco LesVíctor López
Dec 13, 2019·Journal of Agricultural and Food Chemistry·Jianhui LiuZhen-Yu Chen
Feb 12, 2021·Journal of Applied Microbiology·Ebenezeri Erasto NgowiDong-Dong Wu
Jun 19, 2021·Critical Reviews in Food Science and Nutrition·Yunxiao GaoYilin You
Jun 16, 2021·European Journal of Pharmacology·Omnia A NourGhada M Suddek
Jul 3, 2021·Cells·María Ángeles Martín, Sonia Ramos
Jul 29, 2021·Preventive Nutrition and Food Science·Jarinyaporn NaowabootSuvara Wattanapitayakul
Jul 30, 2021·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Martina S SavovaMilen I Georgiev
Aug 2, 2020·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Jiao SongDingkun Zhang
Oct 23, 2018·Journal of Agricultural and Food Chemistry·Berner Andrée Sandoval-RamírezRosa Solà
Oct 12, 2021·Annual Review of Nutrition·Lucas J OsbornJ Mark Brown
Dec 18, 2021·Diabetic Medicine : a Journal of the British Diabetic Association·Sunday O OyedemiShanta J Persaud

❮ Previous
Next ❯

Methods Mentioned

BETA
PCA
Assay
biopsies
ELISA

Related Concepts

Related Feeds

Biomarkers for Type 2 Diabetes

Biomarkers can help understand chronic diseases and assist in risk prediction for prevention and early detection of diseases. Here is the latest research on biomarkers in type 2 diabetes, a disease in which the body is unable to produce or properly use insulin.