Effect of proximal axotomy on GAP-43 expression in cortical neurons in the mouse

Brain Research
E J ElliottP S Fishman

Abstract

As an approach to understanding why central neurons fail to regenerate, we have studied the response to proximal axotomy of transcallosal neurons of the cerebral cortex of the mouse. Anatomical studies have indicated only very slight regenerative responses by this population of cortical neurons. To further examine the regenerative response of these cells, we have looked by in situ hybridization at the expression of GAP-43 mRNA following axotomy caused by a stab wound delivered within about 200 microm to 1.25 mm of the cell body. Axotomized transcallosal neurons were compared with near-by unaxotomized transcallosal neurons, as well as with distant unaxotomized cortical neurons in the contralateral hemisphere. All three populations of neurons had been pre-labeled with Fluoro-Gold to allow identification. No up-regulation of GAP-43 mRNA above background levels was detected for axotomized cortical neurons at 1, 3 or 7 days after injury. In contrast, increases in mean silver grain density of up to 8-fold were measured in axotomized spinal cord motor neurons used as positive controls. Thus, as a population, the transcallosal cortical pyramidal neurons did not show a significant regenerative response, as monitored by GAP-43 upregulati...Continue Reading

Citations

Aug 12, 1999·The Journal of Comparative Neurology·R B IllingR Laszig
Apr 10, 2002·Journal of Neurotrauma·Florencia LabombardaAlejandro F de Nicola
May 4, 1999·Journal of Neurotrauma·E J ElliottP S Fishman
Jun 10, 2003·The Journal of Comparative Neurology·Bryan C HainsStephen G Waxman
Aug 21, 2009·Cellular and Molecular Neurobiology·Maria MeyerAlejandro F De Nicola

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