Effect of pulsatile growth hormone administration to the growth-restricted fetal sheep on somatotrophic axis gene expression in fetal and placental tissues

American Journal of Physiology. Endocrinology and Metabolism
Frank H BloomfieldJane E Harding

Abstract

We have previously reported (Bauer MK, Breier BH, Bloomfield FH, Jensen EC, Gluckman PD, and Harding JE. J Endocrinol 177: 83-92, 2003) that a chronic pulsatile infusion of growth hormone (GH) to intrauterine growth-restricted (IUGR) ovine fetuses increased fetal circulating IGF-I levels without increasing fetal growth. We hypothesized a cortisol-induced upregulation of fetal hepatic GH receptor (GH-R) mRNA levels, secondary increases in IGF-I mRNA levels, and circulating IGF-I levels, but a downregulation of the type I IGF receptor (IGF-IR) as an explanation. We, therefore, measured mRNA levels of genes of the somatotrophic axis by real-time RT-PCR in fetal and placental tissues of fetuses with IUGR (induced by uteroplacental embolization from 110- to 116-days gestation) that received either a pulsatile infusion of GH (total dose 3.5 mg/day) or vehicle from 117-126 days and in control fetuses (n = 5 per group). Tissues were collected at 127 days (term, 145 days). Fetal cortisol concentrations were significantly increased in IUGR fetuses. However, in liver, GH-R, but not IGF-I or IGF-IR, mRNA levels were decreased in both IUGR groups. In contrast, in placenta, GH-R, IGF-I, and IGF-IR expression were increased in IUGR vehicle-in...Continue Reading

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Citations

Jun 4, 2011·Reproduction, Fertility, and Development·Ellen C JensenMaureen Keller-Wood
Oct 7, 2008·Gene Expression Patterns : GEP·Seth A ReiniMaureen Keller-Wood
Feb 19, 2008·Best Practice & Research. Clinical Endocrinology & Metabolism·Primus-E Mullis, Paolo Tonella
Dec 20, 2014·Placenta·A L FowdenO R Vaughan
Feb 2, 2010·Journal of Animal Science·J M KochM E Wilson

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