Abstract
We report the effect of aqueous, ethanol, and methanol solvent leaf extracts of Punica granatum on innate immune mechanisms, such as phagocytosis activity, respiratory burst activity, alternative complement activity, lysozyme activity and functional immunity in terms of percentage cumulative mortality and Relative Percent Survival (RPS) in olive flounder Paralichthys olivaceus naturally infected with lymphocystis disease virus (LDV) after 8 weeks. Infected fish were intraperitoneally administered with 0, 5, 50, and 100 mg kg(-1) body weight of solvent extracts. In groups treated with 50 and 100 mg kg(-1) body weight, the chosen innate immune parameters significantly increased after 8 weeks when compared to 0 mg kg(-1) dose, but not with 5 mg kg(-1). Administration of P. granatum solvent extracts for 8 weeks significantly reduced the percentage mortality with the consequent increase in RPS. The results suggest that intraperitoneal administration of the leaf extracts of P. granatum at 50 or 100 mg kg(-1) dose clearly enhance the innate immune responses and disease resistance after 8 weeks in P. olivaceus against natural LDV infection.
Citations
Apr 27, 2011·Fish Physiology and Biochemistry·Mahdi BanaeeGolam Reza Rafei
Dec 3, 2014·Fish & Shellfish Immunology·Chandrasekar JagruthiRamasamy Harikrishnan
Jan 12, 2013·Developmental and Comparative Immunology·Chen SunShicui Zhang
Aug 22, 2012·Fish & Shellfish Immunology·Ramasamy HarikrishnanMoon-Soo Heo
May 26, 2012·Fish & Shellfish Immunology·Ramasamy HarikrishnanMoon-Soo Heo
Apr 20, 2016·Veterinary Immunology and Immunopathology·Xia ZhouXuegang Li
Feb 24, 2015·Fish & Shellfish Immunology·Nagarajan Balachandran DhayanithiRamasamy Harikrishnan
Dec 25, 2012·Fish & Shellfish Immunology·Saurav KumarKundan Kumar
Jun 24, 2014·Fish & Shellfish Immunology·Sannasi Muthu AnbazahanRamasamy Harikrishnan
Apr 12, 2012·Experimental Parasitology·Ramasamy HarikrishnanMoon-Soo Heo
Nov 5, 2016·Fish & Shellfish Immunology·Hadiseh DadrasAmin Golpour
May 2, 2019·Veterinary World·Omita YengkhomR Dinakaran Michael
Feb 13, 2017·Archives of Virology·Yeon Jung ParkTae-Jin Choi
Jun 9, 2018·Frontiers in Pharmacology·Dongdong WangAtanas G Atanasov
Oct 30, 2020·Research and Reports in Tropical Medicine·Khalid H AbozeidMohamed M Shohayeb
Oct 25, 2021·Journal of Applied Microbiology·Altiery Felix E SilvaCarlos E Copatti