PMID: 39172May 25, 1979

Effect of purified phospholipases on the binding of tetrodotoxin to axon plasma membrane

The Journal of Membrane Biology
G K Chacko


The role of phospholipids in the binding of [3H]tetrodotoxin to garfish olfactory nerve axon plasma membrane was studied by the use of purified phospholipases. Treatment of the membranes with low concentrations of either phospholipase A2 (Crotalus adamanteus and Naja naja) or phospholipase C (Bacillus cereus and Clostridium perfringens) resulted in a marked reduction in tetrodotoxin binding activity. A 90% reduction in the activity occurred with about 45% hydrolysis of membrane phospholipids by phospholipase A2, and with phospholipase C the lipid hydrolysis was about 60--70% for a 70--80% reduction in the binding activity. Phospholipase C from B. cereus and Cl. perfringens had similar inhibitory effects. Bovine serum albumin protected the tetrodotoxin binding activity of the membrane from the inhibitory effect of phospholipase A2 but not from that of phospholipase C. In the presence of albumin about 25% of the membrane phospholipids remained unhydrolyzed by phospholipase A2. It is suggested that these unhydrolyzed phospholipids are in a physical state different from the rest of the membrane phospholipids and that these include the phospholipids which are directly related to the tetrodotoxin binding component. It is concluded th...Continue Reading


Dec 16, 1975·Biochemistry·M BalernaM Lazdunski
Aug 4, 1976·Biochimica Et Biophysica Acta·G K ChackoD E Goldman
Mar 24, 1975·Biochimica Et Biophysica Acta·M A Wells
Jun 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·W S AgnewM A Raftery
Jan 1, 1977·Reviews of Physiology, Biochemistry and Pharmacology·J M Ritchie, R B Rogart
Aug 12, 1976·Nature·A G Lee
Oct 1, 1974·Physiological Reviews·T Narahashi
Feb 27, 1973·Biochimica Et Biophysica Acta·F V BarnolaG Camejo
Apr 3, 1973·Biochimica Et Biophysica Acta·R Coleman
Jun 1, 1970·Proceedings of the National Academy of Sciences of the United States of America·B F TrumpA U Arstila
Dec 10, 1974·Biochimica Et Biophysica Acta·G K ChackoD E Goldman
Dec 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·T I Benzer, M A Raftery
Oct 4, 1972·Nature: New Biology·A M CookM Ishijimi
May 9, 1972·Biochimica Et Biophysica Acta·D R Hafemann
Jul 5, 1958·Nature·A S McFARLANE
Aug 1, 1959·Canadian Journal of Biochemistry and Physiology·E G BLIGH, W J DYER

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Plasma Membrane
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