Effect of pycnogenol on glucose transport in mature 3T3-L1 adipocytes

Phytotherapy Research : PTR
Hee-Hyun LeeBoo-Yong Lee

Abstract

Pycnogenol, a procyanidins-enriched extract of Pinus maritima bark, possesses antidiabetic properties, which improves the altered parameters of glucose metabolism that are associated with type 2 diabetes mellitus (T2DM). Since the insulin-stimulated antidiabetic activities of natural bioactive compounds are mediated by GLUT4 via the phosphatidylinositol-3-kinase (PI3K) and/or p38 mitogen activated protein kinase (p38-MAPK) pathway, the effects of pycnogenol were examined on the molecular mechanism of glucose uptake by the glucose transport system. 3T3-L1 adipocytes were treated with various concentrations of pycnogenol, and glucose uptake was examined using a non-radioisotope enzymatic assay and by molecular events associated with the glucose transport system using semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR). The results show that pycnogenol increased glucose uptake in fully differentiated 3T3-L1 adipocytes and increased the relative abundance of both GLUT4 and Akt mRNAs through the PI3K pathway in a dose dependent manner. Furthermore, pycnogenol restored the PI3K antagonist-induced inhibition of glucose uptake in the presence of wartmannin, an inhibitor of the PI3K. Overall, these results indicat...Continue Reading

References

Sep 13, 1991·Journal of Immunological Methods·N W RoehmA L Glasebrook
Mar 28, 1995·Biochemical and Biophysical Research Communications·J S ElmendorfJ Turinsky
Apr 2, 2002·Biochemical and Biophysical Research Communications·Reiko KameiShinzo Oikawa
May 9, 2002·International Journal of Clinical Pharmacology and Therapeutics·P Rohdewald
Nov 21, 2002·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·L Michelle FurtadoAmira Klip
Dec 25, 2002·International Ophthalmology·F Schönlau, P Rohdewald
Sep 2, 2003·Cytokine & Growth Factor Reviews·Hong Ruan, Harvey F Lodish
Apr 15, 2004·Endocrine Reviews·Robert T WatsonJeffrey E Pessin
Oct 4, 2006·Biological & Pharmaceutical Bulletin·Juntipa PurintrapibanNeil E Forsberg

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Citations

Mar 29, 2014·Molecular Nutrition & Food Research·Chia-Yu HsuChing-Feng Weng
Dec 3, 2014·Antiviral Research·Sayeh EzzikouriKyoko Tsukiyama-Kohara
May 18, 2016·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Xiaoke ZhengWeisheng Feng
May 4, 2010·Chemico-biological Interactions·Kehkashan ParveenWaseem A Siddiqui
Nov 17, 2015·Nutrients·Charlotte GrootaertJohn Van Camp
Jun 13, 2014·Phytotherapy Research : PTR·Gökçe TanerNurşen Başaran
Jul 30, 2021·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Tapan BehlDanut-Eugeniu Mosteanu

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