Effect of pyridoxine deficiency on aromatic L-amino acid decarboxylase in adult rat brain

Experimental Brain Research
Y L Siow, K Dakshinamurti

Abstract

We have investigated the effect of pyridoxine deficiency on aromatic L-amino acid decarboxylase (AADC) using both dihydroxyphenylalanine (DOPA) and 5-hydroxytryptophan (5HTP) as substrates in the rat brain. The activity ratios of DOPA decarboxylase/5HTP decarboxylase measured under optimal substrate and cofactor concentrations were different in the cerebellum, cerebral cortex, corpus striatum and hypothalamus of the normal rat. In pyridoxine deficiency, there were no parallel decreases in DOPA and 5HTP decarboxylase activities in various brain regions. Dialysis of brain homogenates, in the presence and absence of hydroxylamine, resulted in a total or near total loss of 5HTP decarboxylase activity compared to DOPA decarboxylase activity, indicating that pyridoxal phosphate may be more tightly bound to DOPA decarboxylase than to 5HTP decarboxylase. These results, indicating that pyridoxine deficiency has differential effects on the activity of AADC, are consistent with our earlier observation of non-parallel changes in dopamine and serotonin content in various brain regions of the pyridoxine-deficient rat.

Citations

May 10, 1990·Molecular and Cellular Biochemistry·Y L Siow, K Dakshinamurti
Jan 19, 1990·Klinische Wochenschrift·Krishnamurti DakshinamurtiD Bonke
Jun 11, 2010·Molecular Biology Reports·Ioanna ChalatsaDido Vassilacopoulou
Nov 8, 1988·Brain Research·M ViswanathanK Dakshinamurti
Oct 1, 1991·Neuroscience Research·T Nagatsu
Dec 7, 2011·Proceedings of the National Academy of Sciences of the United States of America·Giorgio GiardinaFrancesca Cutruzzolà
Sep 12, 1995·Proceedings of the National Academy of Sciences of the United States of America·F RorsmanO Kämpe
Jan 1, 1990·Annals of the New York Academy of Sciences·K DakshinamurtiB Bolster
Sep 1, 1996·Neurochemical Research·M D BerryA A Boulton
Feb 18, 2016·Nutrition Reviews·Emanuela PanniaG Harvey Anderson
Feb 24, 2009·Molecular Genetics and Metabolism·George F G AllenSimon J R Heales
Jun 10, 2011·Journal of Neurochemistry·Ewa BromekWładysława A Daniel
Jan 1, 1988·Neuroscience and Biobehavioral Reviews·K DakshinamurtiY L Siow
Aug 19, 2015·Canadian Journal of Physiology and Pharmacology·Shyamala Dakshinamurti, Krishnamurti Dakshinamurti
Aug 14, 1992·Biochemical and Biophysical Research Communications·Y KawasakiH Kagamiyama
Nov 6, 2012·Journal of Geriatric Psychiatry and Neurology·Wen-Harn PanWei J Chen
Jun 27, 2018·Pharmacological Reviews·Raul R GainetdinovMark D Berry
Oct 10, 2014·Journal of Nutritional Science and Vitaminology·Chiaki HiratsukaKatsumi Shibata

Related Concepts

Metazoa
Aromatic-L-Amino-Acid Decarboxylases
Brain
Brain Chemistry
DOPA decarboxylase
5-Hydroxytryptophan
August Rats
Vitamin B 6 Deficiency
Rats, Laboratory

Related Feeds

Basal Ganglia

Basal Ganglia are a group of subcortical nuclei in the brain associated with control of voluntary motor movements, procedural and habit learning, emotion, and cognition. Here is the latest research.