Effect of pyrrolidine dithiocarbamate on hepatic vascular stress gene expression during ischemia and reperfusion

European Journal of Pharmacology
Chan-Ho LeeSun-Mee Lee

Abstract

Pyrrolidine dithiocarbamate, an antioxidant and a potent inhibitor of nuclear factor-kappa B (NF-kappaB), is known to have protective effect against ischemia and reperfusion injury. This study examined the cytoprotective mechanism of pyrrolidine dithiocarbamate against the microcirculatory failure caused by hepatic ischemia and reperfusion. Rats were subjected to 60 min of hepatic ischemia followed by 5 h of reperfusion. Pyrrolidine dithiocarbamate (100 mg/kg) or the vehicle was administered intraperitoneally 24 h before ischemia. The level of serum aminotransferases and hepatic lipid peroxides significantly increased, and the glutathione contents fell in the ischemia/reperfusion group. Pyrrolidine dithiocarbamate prevented the increase in the level of serum enzymes and hepatic lipid peroxides, and the decrease in the glutathione contents. The NF-kappaB DNA-binding activity was inhibited by a pre-treatment with pyrrolidine dithiocarbamate. Ischemia and reperfusion significantly increased the mRNA expression of the endothelin-1 and endothelin ET(B) receptor, which was prevented by pyrrolidine dithiocarbamate. There were significant increases in the mRNA expressions of inducible nitric oxide synthase, tumor necrosis factor-alpha,...Continue Reading

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Citations

Jun 14, 2014·Journal of Photochemistry and Photobiology. B, Biology·Ana L M IvanRúbia Casagrande
Apr 12, 2013·Oxidative Medicine and Cellular Longevity·Paulina KleniewskaAnna Goraca
Jul 5, 2015·Toxicon : Official Journal of the International Society on Toxinology·Camila R FerrazWaldiceu A Verri
May 13, 2010·Bioscience, Biotechnology, and Biochemistry·Yuko KitamuraShosuke Kojo
May 26, 2012·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·Ashley M StokesWarren S Warren

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