Abstract
This study aims to explore the effect and mechanism of pyruvate kinase M2 (PKM2) on chemotherapy resistance of estrogen receptor-positive breast cancer (ER BC) by regulating aerobic glycolysis. The expression of PKM2 in ER BC MCF-7 cells, T47D cells and MCF-7/ADR cells (which are subject to adriamycin/ADR induction) were determined by quantitative real-time PCR and western blot. MCF-7/ADR (M/A) cells were grouped into blank group (M/A), negative group (M/A+NC), low expression of PKM2 group (M/A+si-PKM2 group), overexpression of PKM2 group (M/A+PKM2 group) and glycolysis inhibition group (M/A+PKM2+2-DG group). Quantitative real-time PCR and western blot were applied to measure the expressions of PKM2, multidrug resistance, and glutathione-S-transferase π. Glucose and lactic acid kit was used to detect the amount of glucose uptake and lactic production. Cell variability, clone formation ability, and cell apoptosis were respectively measured by MTT, clone formation assay, and flow cytometry. Transwell assay and scratch assay were applied for cell invasion and migration ability. By overexpressing PKM2 in MCF-7 and T47D cells and using 2-DG, the effect on sensitivity of adriamycin amycin was explored. MCF-7/ADR cells have both eleva...Continue Reading
References
May 29, 2010·Cancer Science·Hua-shan ShiYu-Quan Wei
Jul 2, 2010·Cancer Chemotherapy and Pharmacology·Haibin XiTheodore J Lampidis
Sep 15, 2010·Proceedings of the National Academy of Sciences of the United States of America·S Neil VaishnaviMarcus E Raichle
Sep 15, 2010·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Torsten O NielsenMatthew J Ellis
Nov 10, 2010·Breast Cancer Research : BCR·Edgardo Rivera, Henry Gomez
Jan 19, 2011·The Journal of Experimental Medicine·Amparo WolfAbhijit Guha
May 4, 2011·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Bernd GerberUNKNOWN German Breast Group Investigators
Jun 28, 2011·Molecular Cell·Lei LvQun-Ying Lei
Oct 12, 2011·Annual Review of Cell and Developmental Biology·Sophia Y Lunt, Matthew G Vander Heiden
Nov 8, 2011·Nature·Weiwei YangZhimin Lu
Feb 7, 2012·Molecular Cell·Xueliang GaoZhi-Ren Liu
Jun 2, 2012·Nature Reviews. Drug Discovery·Bedrich L EckhardtRobin L Anderson
Jul 25, 2012·Trends in Endocrinology and Metabolism : TEM·Weibo Luo, Gregg L Semenza
Aug 21, 2012·Cell·Weiwei YangZhimin Lu
Nov 6, 2012·Molecular Cell·Weiwei YangZhimin Lu
Nov 28, 2012·Nature Cell Biology·Weiwei YangZhimin Lu
Sep 10, 2013·Cell Cycle·Weiwei Yang, Zhimin Lu
Oct 12, 2013·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Eva M Palsson-McDermott, Luke A J O'Neill
Dec 10, 2013·Molecular Cell·Yuhui JiangZhimin Lu
Mar 1, 2014·IUBMB Life·Haibin XiTheodore J Lampidis
Jul 16, 2014·Nature Communications·Liangchun YangDaolin Tang
Sep 19, 2014·British Journal of Cancer·C PapadakiJ Souglakos
Mar 5, 2015·Biochemical and Biophysical Research Communications·Meredith L ReesHeinrich Taegtmeyer
Oct 27, 2015·JAMA : the Journal of the American Medical Association·Kevin C OeffingerUNKNOWN American Cancer Society
Jun 18, 2016·Clinical & Translational Oncology : Official Publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico·J A MestresP Sanchez-Rovira
Sep 7, 2016·Yonsei Medical Journal·Sujuan YuanQi Zhang
Citations
May 30, 2019·Journal of Cellular Physiology·Xin GeDongwei Dou
Aug 19, 2020·Cancers·Elizabeth VargheseDietrich Büsselberg
Jan 9, 2021·Neoplasia : an International Journal for Oncology Research·Fabrizio Marcucci, Cristiano Rumio
Sep 8, 2019·Drug Resistance Updates : Reviews and Commentaries in Antimicrobial and Anticancer Chemotherapy·Teodora Alexa-StratulatChiara Riganti
Jan 30, 2021·Molecular and Cellular Biochemistry·Zhi YuYingying Tang