Effect of radiologic contrast media on cell volume regulation in rabbit proximal renal tubules

Academic Radiology
Hilde Kanli GaltungKjell S Sakariassen

Abstract

Most radiographic contrast media are hyperosmotic and able to shrink cells with which they are in contact. The authors studied cell volume control in rabbit proximal renal tubules after incubation with three contrast media: iohexol, ioxaglate, and iodixanol. Proximal renal tubules were isolated from rabbit kidneys. The tubules were exposed to Ringer solutions containing 5% vol/vol iohexol (final osmolality, 330 mOsm), ioxaglate (323 mOsm), iodixanol (305 mOsm), or mannitol (control solutions with identical osmolalities), and tubule volumes were monitored. After 2 hours of incubation, the tubules were stimulated with a hyposmotic Ringer solution (165 mOsm). Three groups of 10 experiments were performed. All solutions induced cell shrinkage (8.3%+/-3.8 [standard error] to 15.4%+/-0.5), which was completely or partly reversible in most experiments (volume increase, 44.8%+/-14.7 to 149.9%+/-107.3) but not those with iohexol and iodixanol. With exposure to the hyposmotic solution, the cells swelled by 11.0%+/-1.8 to 39.7%+/-4.8. In general, the tubules that had been exposed to the most hyperosmotic solution swelled the most. Those exposed to contrast media showed less swelling than the mannitol-exposed controls. In all control exper...Continue Reading

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Citations

Aug 21, 2002·Academic Radiology·Hilde Kanli GaltungHaakon B Benestad
Jan 24, 2012·European Heart Journal·Erdmann SeeligerPontus B Persson
Feb 26, 2011·Clinical and Experimental Pharmacology & Physiology·Mauricio M Sendeski
Sep 15, 2012·The Tohoku Journal of Experimental Medicine·Sejoong KimJin Suk Han
Jun 2, 2020·The Journal of Surgical Research·Richard S MangusWilliam C Goggins

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