Effect of receptor specificity of A/Hong Kong/1/68 (H3N2) influenza virus variants on replication and transmission in pigs.

Influenza and Other Respiratory Viruses
Sjouke G M Van PouckeKristien Van Reeth

Abstract

Several arguments plead for an important role of pigs in human influenza ecology, including the similar receptor expression pattern in the respiratory tract of both species. How virus receptor binding specificity affects transmission in pigs, on the other hand, has not been studied so far.   Using recombinant viruses R1-HK, which harbored all genes from the original pandemic virus A/Hong Kong/1/68 (H3N2), and R2-HK, which differed by L226Q and S228G mutations in the hemagglutinin and conversion to an avian-virus-like receptor specificity, we assessed the role of receptor specificity on (i) replication in porcine respiratory explants, (ii) pig-to-pig transmission, and (iii) replication and organ tropism in pigs. In nasal, tracheal, and bronchial explants, we noticed a 10- to 100-fold lower replication of R2-HK compared with R1-HK. In the lung explants, the viruses replicated with comparable efficiency. These observations correlated with the known expression level of Siaα2,3-galactose in these tissues. In the pathogenesis study, virus titers in the respiratory part of the nasal mucosa, the trachea, and the bronchus were in line with the ex vivo results. R2-HK replicated less efficiently in the lungs of pigs than R1-HK, which cont...Continue Reading

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Citations

Sep 5, 2013·Influenza and Other Respiratory Viruses·Renee W Y ChanJohn M Nicholls
Sep 18, 2015·Journal of Virology·Sjouke Van PouckeMikhail Matrosovich
Apr 7, 2017·PloS One·Jose Carlos Mancera GraciaKristien Van Reeth

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Methods Mentioned

BETA
ELISA
genetic modifications

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