PMID: 15374274Nov 1, 1994Paper

Effect of reduced glutathione on mitochondrial parameters of proliferating splenocytes from young and old rats

Archives of Gerontology and Geriatrics
C PieriM Marra

Abstract

The time-dependent changes of mitochondrial membrane potential and mass have been investigated on splenocytes from young, adult and old rats stimulated with Con A in the presence and absence of reduced glutathione (GSH). In addition, the basal level as well as the level of GSH during a 3-day culture period has been determined. No age-dependent changes of cellular GSH content were observed in freshly prepared splenocytes; however, in proliferating cells from old animals the expected increase in GSH levels was delayed. As regards the mitochondrial parameters, their membrane potential and mass were measured by means of the fluorescent probes rhodamine-123 (Rh-123) and nonyl acridine orange (NAO), respectively, and flow cytometry. During aging and with time of culture, an increased number of cells showed depolarization and loss of mitochondrial mass. This age-dependent impairment was completely prevented by addition of GSH to the culture medium, which resulted in a sharp increase in intracellular GSH. The present findings support the view that an impairment of the antioxidant defense system may be responsible for the damage observed in the mitochondria of proliferating splenocytes from old animals.

References

May 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·M SuthanthiranA Meister
Jan 1, 1988·Annual Review of Pharmacology and Toxicology·I A CotgreaveS Orrenius
Apr 1, 1987·Mechanisms of Ageing and Development·T FurukawaJ B Blumberg
Jan 1, 1986·Cellular Immunology·R K Fidelus, M F Tsan
Jan 1, 1986·Biochemical Pharmacology·D J Reed
Aug 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·G L Jensen, A Meister
Apr 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·Z DarzynkiewiczM R Melamed
Dec 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·J K Dethmers, A Meister

❮ Previous
Next ❯

Citations

Apr 1, 1996·Journal of Photochemistry and Photobiology. B, Biology·J Plásek, K Sigler
Mar 17, 2001·Archives of Gerontology and Geriatrics·L MalaguarneraA Messina
Dec 13, 2005·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Werner J H KoopmanPeter H G M Willems

❮ Previous
Next ❯

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.