Effect of rejuvenation and frozen storage on 42-day-old AS-1 RBCs

Transfusion
I O SzymanskiG S Johnson

Abstract

The FDA has approved a 42-day storage period for RBCs stored in ADSOL (AS-1). This study was undertaken to provide data for the FDA about the feasibility of salvaging AS-1 RBCs at the end of their storage period by rejuvenation and freezing. The investigation, consisting of a study (n = 10) and control (n = 6) arm, was carried out in two centers. In both centers, eight healthy volunteers donated a unit (450 mL) of whole blood. The RBC concentrates were stored at 4 degrees C in AS-1 for 42 days. The study units were rejuvenated, whereas the control units were not. All units were stored frozen at -80 degrees C, then deglycerolized and kept for an additional 24 hours at 4 degrees C. After the 42-day storage period, ATP had declined to 62 percent of the original value, 2,3 DPG was zero, and MCV was significantly larger than that of fresh RBCS: Following rejuvenation and deglycerolization, the mean ATP level was 141 percent, the mean 2,3 DPG level was 109 percent, and the MCV was normal. The freeze-thaw-wash recovery of the rejuvenated and nonrejuvenated RBCs was similar, 88.4 and 84.0 percent, respectively. There was no difference in hypoxanthine, inosine, and uric acid levels in the rejuvenated and nonrejuvenated units, which indi...Continue Reading

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Citations

Aug 25, 2009·Current Opinion in Hematology·Mark T Gladwin, Daniel B Kim-Shapiro
Nov 18, 2003·Transfusion·William B LockwoodAlan D Gray
Jan 21, 2009·Vox Sanguinis·T PottgiesserY O Schumacher
Jun 5, 2018·Vox Sanguinis·H AujlaUNKNOWN REDJUVENATE Investigators

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