Effect of relaxin on TGF-beta1 expression in cultured vaginal fibroblasts from women with stress urinary incontinence

Reproductive Sciences
Yan WenBertha Chen

Abstract

The objective of this study is to compare relaxin's effect on transforming growth factor (TGF)- beta1 and latent TGF-beta1-binding protein (LTBP-1) in vaginal fibroblasts from women with stress urinary incontinence (SUI) to continent women (controls) in both phases of the menstrual cycle. Fibroblasts were treated with relaxin. TGF-beta1 levels were measured by enzyme-linked immunosorbent assay. LTBP-1 expression was evaluated by Western blot. In the proliferative phase, total TGF-beta1 level in the supernatant, cells, and extracellular matrix (ECM) of SUI fibroblasts decreased with increasing relaxin concentration (P < .05). Active TGF-beta1 levels increased at a low concentration of relaxin (P < .05) in the supernatant but decreased in the ECM of SUI fibroblasts at high concentration (P < .05). In the secretory phase, total TGF-beta1 levels decreased with relaxin treatment (P < .05) in the supernatant, cells and ECM of both women with SUI and controls. Relaxin decreased the levels of total and active TGF-beta1 in the ECM isolated from SUI vaginal fibroblasts.

References

Oct 1, 1990·American Journal of Respiratory Cell and Molecular Biology·S E McGowan, R McNamer
Nov 10, 1994·The New England Journal of Medicine·W A Border, N A Noble
Jun 15, 1996·Lancet·S R JacksonA J Bailey
Jan 1, 1997·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·J Taipale, J Keski-Oja
Jan 1, 1997·General Pharmacology·D Bani
Apr 18, 1998·The Biochemical Journal·W KafienahA P Hollander
Apr 25, 2000·Obstetrics and Gynecology·D HowardJ A Ashton-Miller
May 4, 2000·The New England Journal of Medicine·G C BlobeH F Lodish
Jun 11, 2002·International Urogynecology Journal and Pelvic Floor Dysfunction·B H ChenM L Polan
Dec 7, 2002·Obstetrics and Gynecology·Carolyn M SampselleIrina Bondarenko
Dec 17, 2002·Journal of Cell Science·Justin P AnnesDaniel B Rifkin
Mar 7, 2003·The New England Journal of Medicine·Guri RortveitUNKNOWN Norwegian EPINCONT Study
Mar 15, 1965·American Journal of Obstetrics and Gynecology·R P BECK, N HSU
Feb 24, 2004·Gastroenterology·John O L Delancey, James A Ashton-Miller
Feb 26, 2004·Neurourology and Urodynamics·Bertha ChenMary Lake Polan
May 20, 2005·American Journal of Obstetrics and Gynecology·Bertha ChenMary Lake Polan
Jun 16, 2005·Annals of the New York Academy of Sciences·Chrishan S SamuelTim D Hewitson
Jun 16, 2005·Annals of the New York Academy of Sciences·Ishanee MookerjeeChrishan S Samuel
Nov 8, 2005·Scandinavian Journal of Primary Health Care·Gitte RohrJakob Kragstrup
Nov 24, 2005·Clinical Medicine & Research·Chrishan S Samuel

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Citations

Jun 3, 2016·British Journal of Pharmacology·C S SamuelR G Bennett
Feb 13, 2013·Medicinal Research Reviews·Valeria CernaroMichele Buemi
Oct 31, 2012·Gynecologic and Obstetric Investigation·Aukje M MeijerinkPaul J Q van der Linden
Dec 6, 2011·The Journal of Obstetrics and Gynaecology Research·Yan WenBertha Chen

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