Abstract
The stability of circulating proteins can be affected by repeated freezing and thawing. The aim of our study was to identify the effect of repeated freezing and thawing on the plasma and serum concentrations of eight proteins [interferon-γ, interleukin (IL)-8, IL-15, IL-17A, matrix metalloproteinase (MMP)-7, tumor necrosis factor-α, vascular endothelial growth factor (VEGF), and VEGF receptor 2 (VEGF-R2)]. We assessed the concentration changes of these proteins in 30 plasma and serum samples subjected to three, four, or five freeze-thaw cycles, and compared these with the concentration changes in the samples that were subjected to two freeze-thaw cycles before analysis. Repeated freezing and thawing by up to five cycles did not modify the plasma and serum concentrations of interferon-γ, IL-8, and VEGF-R2, while levels of MMP-7, tumor necrosis factor-α, and VEGF were significantly changed in both plasma and serum samples. Moreover, MMP-7 and VEGF concentrations tended to increase with freeze-thaw cycles. They were more elevated in plasma samples (up to about 15%) than in serum samples (up to about 7%), suggesting that serum is the preferred sample type for the analysis of circulating proteins. This is the first report on the eff...Continue Reading
References
Aug 30, 1992·Journal of Immunological Methods·P W ThavasuF R Balkwill
Aug 6, 2000·Stroke; a Journal of Cerebral Circulation·M SlevinJ Gaffney
Mar 10, 2001·International Journal of Gynecological Cancer : Official Journal of the International Gynecological Cancer Society·H.-S. MoonB. H. Woo
Nov 17, 2001·Arthritis and Rheumatism·N IshiguroA R Poole
Dec 19, 2002·Molecular & Cellular Proteomics : MCP·N Leigh Anderson, Norman G Anderson
Jul 23, 2003·Proteomics·Rembert PieperSandra Steiner
Dec 29, 2004·Journal of Pharmaceutical and Biomedical Analysis·Chad A RayRobert A Dean
Jul 20, 2007·Clinical Chemistry and Laboratory Medicine : CCLM·Christine ChaigneauFotini Betsou
May 2, 2008·PLoS Medicine·Ivan O RosasNaftali Kaminski
Jun 24, 2008·Osteoarthritis and Cartilage·S M LingL Ferrucci
Sep 23, 2008·Lung·Fisun KaradagHatice Ozcan
Oct 22, 2008·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Hui-Ching ChuangJiin-Haur Chuang
Sep 10, 2009·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Roopali RoyMarsha A Moses
Jun 17, 2010·International Archives of Medicine·Aamir ShahzadGottfried Köhler
Dec 2, 2010·Clinical Chemistry and Laboratory Medicine : CCLM·Kalle KisandAgu Tamm
Jan 21, 2012·American Journal of Clinical Pathology·Shine-Young KimDo Youn Park
Jan 8, 2013·Molecular Medicine Reports·Guang-Hong GuoYa-Ping Tian
Aug 7, 2013·Gynecologic Oncology·Yu-Li ChenChi-An Chen
Mar 4, 2014·PloS One·Michaela BreierAndreas Lechner
Oct 1, 2012·Biopreservation and Biobanking·Mohsen Azimi-NezhadSophie Visvikis Siest
Oct 11, 2014·Arthritis & Rheumatology·Juan ChenDana P Ascherman
Mar 7, 2015·Asian Pacific Journal of Cancer Prevention : APJCP·Halide KayaAbdurrahman Senyigit
Citations
Oct 26, 2016·EBioMedicine·Stefan EnrothUlf Gyllensten
Oct 26, 2016·EBioMedicine·Jose A Cancelas, Neeta Rugg
Oct 19, 2017·Clinical Chemistry and Laboratory Medicine : CCLM·Qiujin ShenUlf Landegren
Sep 9, 2017·Frontiers in Zoology·Arne HegemannKevin D Matson
Apr 14, 2017·RMD Open·Nicola GoodfellowRaashid Luqmani
May 28, 2019·Biomolecules·Manoj KhadkaEric A Ortlund
Sep 17, 2019·Clinical Chemistry and Laboratory Medicine : CCLM·Mario PlebaniTommaso Trenti
Nov 9, 2019·Clinical Chemistry and Laboratory Medicine : CCLM·Joachim K W Pum
Jun 11, 2019·Prehospital Emergency Care : Official Journal of the National Association of EMS Physicians and the National Association of State EMS Directors·Emily B BrantChristopher W Seymour
Aug 8, 2020·Journal of Clinical Microbiology·Francesco BisogninPaola Dal Monte
Dec 20, 2018·Frontiers in Oncology·Kelly Yi Ping LiuCatherine F Poh
Jul 18, 2018·PeerJ·Qiuxia HanHanyu Zhu
Jun 17, 2020·Wellcome Open Research·Ursula K RohlwinkUNKNOWN Tuberculous Meningitis International Research Consortium
Jan 6, 2017·Osong Public Health and Research Perspectives·Jae-Eun LeeShine Young Kim
Nov 7, 2019·PloS One·Ditte S JonescoKim Henriksen
Jan 16, 2020·Sensors·Young Ju LeeGi-Ja Lee
Feb 1, 2019·Science Translational Medicine·Lea C BerkhoutTheo Rispens
May 23, 2018·BMC Nephrology·Christopher E AndersonJing Chen
Sep 1, 2019·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Wenxin XuRupal S Bhatt
Feb 29, 2020·Cytokine·Ulrik StervboNina Babel
Apr 21, 2021·Journal of Immunological Methods·Winnie Charlotte Pedersen MortensenMorten Rune Blichfeldt-Eckhardt
May 11, 2021·Alzheimer's & Dementia : Diagnosis, Assessment & Disease Monitoring·Fernando BartolomeEva Carro
Jun 12, 2021·Advanced Science·Chao LiuGuozhen Liu
Aug 7, 2021·The Neuroscientist : a Review Journal Bringing Neurobiology, Neurology and Psychiatry·Emilia GiannellaGiulia Sancesario
Jul 6, 2021·Clinical Chemistry and Laboratory Medicine : CCLM·Elena Revuelta-LópezAntoni Bayes-Genis
May 28, 2021·Applied Spectroscopy·James M CameronMatthew J Baker