Effect of Repeated Freezing and Thawing on Biomarker Stability in Plasma and Serum Samples

Osong Public Health and Research Perspectives
Jae-Eun LeeSo-Youn Shin

Abstract

The stability of circulating proteins can be affected by repeated freezing and thawing. The aim of our study was to identify the effect of repeated freezing and thawing on the plasma and serum concentrations of eight proteins [interferon-γ, interleukin (IL)-8, IL-15, IL-17A, matrix metalloproteinase (MMP)-7, tumor necrosis factor-α, vascular endothelial growth factor (VEGF), and VEGF receptor 2 (VEGF-R2)]. We assessed the concentration changes of these proteins in 30 plasma and serum samples subjected to three, four, or five freeze-thaw cycles, and compared these with the concentration changes in the samples that were subjected to two freeze-thaw cycles before analysis. Repeated freezing and thawing by up to five cycles did not modify the plasma and serum concentrations of interferon-γ, IL-8, and VEGF-R2, while levels of MMP-7, tumor necrosis factor-α, and VEGF were significantly changed in both plasma and serum samples. Moreover, MMP-7 and VEGF concentrations tended to increase with freeze-thaw cycles. They were more elevated in plasma samples (up to about 15%) than in serum samples (up to about 7%), suggesting that serum is the preferred sample type for the analysis of circulating proteins. This is the first report on the eff...Continue Reading

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Citations

Apr 14, 2017·RMD Open·Nicola GoodfellowRaashid Luqmani
Sep 17, 2019·Clinical Chemistry and Laboratory Medicine : CCLM·Mario PlebaniTommaso Trenti
Nov 9, 2019·Clinical Chemistry and Laboratory Medicine : CCLM·Joachim K W Pum
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Jun 17, 2020·Wellcome Open Research·Ursula K RohlwinkUNKNOWN Tuberculous Meningitis International Research Consortium
Jan 6, 2017·Osong Public Health and Research Perspectives·Jae-Eun LeeShine Young Kim
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Methods Mentioned

BETA
Assay
ELISA

Software Mentioned

SPSS

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