Effect of reserpine on Pseudomonas aeruginosa quorum sensing mediated virulence factors and biofilm formation

Biofouling
Debaprasad ParaiSamir Kumar Mukherjee

Abstract

This study aimed to evaluate the effect of reserpine, a plant-derived indole-alkaloid, against Pseudomonas aeruginosa PAO1 biofilms. The anti-biofilm activity of reserpine was evaluated by crystal violet staining, MTT assay, confocal laser scanning microscopy and scanning electron microscopy. Reserpine effects were also assessed by qRT-PCR of quorum sensing (QS)-regulated genes and biochemical quantification of the QS-mediated virulence factors pyocyanin, rhamnolipids, proteases and elastases. Reserpine reduced biofilm formation, cell motility, virulence factor production, and QS-controlled gene expression. Additionally, molecular docking analysis for AHL synthase LasI and QS transcriptional regulators LasR/MvfR revealed a plausible molecular mechanisms of reserpine QS inhibition. These findings provide insights into the underlying mode of action of reserpine, which may be useful in the development of new drugs against biofilm-related infections.

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Citations

Jul 18, 2019·Natural Product Research·T UshanthikaJ Vijayashree Priyadharsini
Dec 11, 2019·Applied Microbiology and Biotechnology·Fazlurrahman KhanYoung-Mog Kim
Apr 13, 2020·Journal of Applied Microbiology·Natalia I Di MarcoCecilia S M Lucero-Estrada
Nov 27, 2019·Applied Microbiology and Biotechnology·Fazlurrahman KhanYoung-Mog Kim

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Methods Mentioned

BETA
scanning electron microscopy
PCR
X-ray

Software Mentioned

Molegro molecular viewer
PyMOL Molecular Graphics System
Leica application suite X ( LAS X )
Discovery Visualizer
PyMOL
smartSEM
Patchdock
LasX
GraphPad Prism
GraphPad

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