Effect of resistance exercise under conditions of reduced blood insulin on AMPKα Ser485/491 inhibitory phosphorylation and AMPK pathway activation

American Journal of Physiology. Regulatory, Integrative and Comparative Physiology
Kohei KidoSatoshi Fujita

Abstract

Insulin stimulates skeletal muscle glucose uptake via activation of the protein kinase B/Akt (Akt) pathway. Recent studies suggest that insulin downregulates AMP-activated protein kinase (AMPK) activity via Ser485/491 phosphorylation of the AMPK α-subunit. Thus lower blood insulin concentrations may induce AMPK signal activation. Acute exercise is one method to stimulate AMPK activation; however, no study has examined the relationship between blood insulin levels and acute resistance exercise-induced AMPK pathway activation. Based on previous findings, we hypothesized that the acute resistance exercise-induced AMPK pathway activation would be augmented by disruptions in insulin secretion through a decrease in AMPKα Ser485/491 inhibitory phosphorylation. To test the hypothesis, 10-wk-old male Sprague-Dawley rats were administered the toxin streptozotocin (STZ; 55 mg/kg) to destroy the insulin secreting β-cells. Three days postinjection, the right gastrocnemius muscle from STZ and control rats was subjected to resistance exercise by percutaneous electrical stimulation. Animals were killed 0, 1, or 3 h later; activation of the Akt/AMPK and downstream pathways in the muscle tissue was analyzed by Western blotting and real-time PCR....Continue Reading

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Apr 19, 2020·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Di CuiZhen Yan
Oct 21, 2020·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Li-Ni SongJin-Kui Yang

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Methods Mentioned

BETA
ELISA
Protein Assay
electrophoresis
PCR
Assay
nuclear translocation

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