Effect of resveratrol on SNARE proteins expression and insulin resistance in skeletal muscle of diabetic rats

Iranian Journal of Basic Medical Sciences
Azam Rezaei FarimaniSoheila Asadi

Abstract

Soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) complex proteins are involved in membrane trafficking. The expression of isoforms of SNAP-23, syntaxin-4, and VAMP-2 is significantly done in skeletal muscles; they control GLUT4 trafficking. It is believed that type 2 diabetes could be caused by the modifications in the expression of SNARE complex proteins. The purpose of this study was to evaluate the effect of resveratrol on the expression of these proteins in type 2 diabetes. Forty male Wistar rats were selected. Streptozotocin and nicotinamide were applied for the induction of type 2 diabetes. The animals were divided into five groups. Healthy and diabetic groups were set as control; resveratrol (1, 5, and 10 mg/kg body weight) was applied to treat the three groups of diabetic rats for 30 days. Real-time qRT-PCR was applied to evaluate the expression of SNARE complex proteins. There is a link between diabetes and insulin resistance and up-regulation of SNARE proteins expression. Resveratrol improved hyperglycemia and insulin resistance along with a non-significant reduction in the expression of SNARE proteins. Increased expression of SNARE proteins was possibly a compensatory mechanism in respons...Continue Reading

Methods Mentioned

BETA
ELISA
electrophoresis
PCR

Software Mentioned

AlleleID7
SPSS

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