Effect of Reverse End-to-Side (Supercharging) Neurotization in Long Processed Acellular Nerve Allograft in a Rat Model

The Journal of Hand Surgery
Jonathan IsaacsRachel Semus

Abstract

Processed acellular nerve allograft (PNA) has been suggested as a convenient tool for overcoming short and medium nerve defects. Although the clinical implications are unclear, animal data suggest that PNA becomes less effective at longer lengths. Although reverse or supercharging end-to-side nerve transfer may improve the neurotrophic potential in chronically denervated nerve tissue, the application of this strategy to long acellular nerve allograft has not been previously investigated. We hypothesized that supercharging acellular nerve allograft would increase its effective length. Sprague-Dawley and Thy1-green fluorescent protein Sprague-Dawley rats underwent transection of the tibial nerve, followed by immediate repair with 20-, 40-, or 60-mm acellular nerve allografts processed identically to commercially available human acellular nerve allograft (AxoGen, Inc., Alachua, FL) or isograft. Half of the allograft group was supercharged with a reverse end-to-side transfer from the ipsilateral peroneal nerve. At 10 weeks, the reconstructed nerve in the Thy1-green fluorescent rat groups were exposed and examined under a fluorescence-enabled microscope. At 20 weeks, the remaining rats underwent motor testing and tissue harvest for ...Continue Reading

Citations

Nov 15, 2019·Muscle & Nerve·Jonathan IsaacsCamden Kurtz
Aug 28, 2020·Current Reviews in Musculoskeletal Medicine·Andrew Baron, Adam Strohl
Feb 4, 2021·Hand : Official Journal of the American Association for Hand Surgery·Jonathan Isaacs
Apr 24, 2021·Plastic and Reconstructive Surgery·Blair R PetersAmy M Moore
Dec 21, 2020·The Journal of Hand Surgery·Amir Arami, Jayme Augusto Bertelli

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