PMID: 9546650Apr 18, 1998Paper

Effect of reversible reactions on isotope label redistribution--analysis of the pentose phosphate pathway

European Journal of Biochemistry
B D Follstad, Gregory Stephanopoulos

Abstract

The pentose phosphate pathway plays several key roles in metabolism including supply of biosynthetic carbon skeletons and reducing power. Previous research has focused on determining the fluxes through the reactions of this pathway using carbon-labeled substrates and models that make certain assumptions about the reversibility of the transketolase and transaldolase reactions in the nonoxidative pathway. These assumptions, however, have resulted in inconsistencies between the predicted carbon label distributions using these models and those determined experimentally. A general metabolic reaction network model developed in this paper and applied to the pentose phosphate pathway not only incorporates reaction reversibility but also accounts for the effect of individually varying extents of reaction reversibility on labeled carbon fractional enrichment values for intermediate metabolites. In addition, an algorithm is presented that can be used to calculate the three individual transaldolase and transketolase extents of reversibility. The results of this method show that varying extents of reaction reversibility have an observable effect on the metabolite carbon label distributions which can in turn affect flux calculation for other...Continue Reading

Citations

Sep 22, 1999·European Journal of Biochemistry·J C PortaisJ N Barbotin
Jun 6, 2002·European Journal of Biochemistry·Bjarke ChristensenJens Nielsen
Jun 18, 2005·Current Opinion in Biotechnology·Mattheos Koffas, Gregory Stephanopoulos
Dec 19, 2012·Metabolic Engineering·Scott B Crown, Maciek R Antoniewicz
Aug 16, 2003·European Journal of Biochemistry·Maria I KlapaGregory Stephanopoulos
May 12, 2004·Current Opinion in Plant Biology·Jörg SchwenderYair Shachar-Hill
Nov 9, 2005·Phytochemistry·Christian EttenhuberWolfgang Eisenreich
Dec 8, 2014·Microbiology·Christian J SundMargaret M Hurley
Nov 5, 2002·FEMS Microbiology Reviews·Jean-Charles Portais, Anne-Marie Delort
Aug 10, 2000·Metabolic Engineering·G Stephanopoulos
Oct 7, 2003·Annual Review of Biomedical Engineering·Martin L Yarmush, Scott Banta
Apr 6, 2001·Metabolic Engineering·W van WindenS Heijnen
Sep 9, 1999·Tissue Engineering·K LeeM L Yarmush
Aug 9, 2001·Biotechnology and Bioengineering·W A van WindenJ Grievink
Aug 16, 2001·Biotechnology and Bioengineering·M DaunerU Sauer
Jul 3, 2002·The Journal of Biological Chemistry·Billyana C TsvetanovaNeil P J Price
Jul 9, 1999·Biotechnology and Bioengineering·B D FollstadD I Wang
May 22, 2003·The Journal of Biological Chemistry·Jorg SchwenderYair Shachar-Hill
Aug 2, 2008·Prikladnaia biokhimiia i mikrobiologiia·A D KiveroA S Arsen'ev
Apr 1, 1999·Biotechnology and Bioengineering·C Wittmann, E Heinzle
Feb 16, 2000·Journal of Biotechnology·J C PortaisJ N Barbotin
May 29, 2007·Phytochemistry·Doug K AllenJohn B Ohlrogge
Oct 26, 2007·Metabolic Engineering·Huimin Yu, Gregory Stephanopoulos
Nov 15, 2001·Journal of the American Chemical Society·R N GuptaI D Spenser

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