Effect of ribonuclease A and deoxyribonuclease I on immunostaining of Ki-67 in fixed-embedded sections

Pathology, Research and Practice
G SzekeresJ Diebold

Abstract

Immunostaining of the cell cycle-associated Ki-67 antigen was studied on routinely formalin-fixed and paraffin-embedded tissue sections, using the Ki-67-specific monoclonal antibody MIB-1. Immunomorphological analysis of the Ki-67 immunostaining pattern was carried out following tissue pre-treatments including combinations of microwave heating and trypsinization, as well as of ribonuclease and deoxyribonuclease pre-digestion of the sections. The nucleolar Ki-67 immunostaining after slide pre-treatment by microwave heating followed by trypsinization was reduced only by ribonuclease pre-digestion, if this latter was used before heating. Ki-67 immunostaining was not significantly reduced by deoxyribonuclease treatment. We conclude that Ki-67, located in the nucleoli, may be associated there with nucleolar RNA.

References

Jun 1, 1991·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·S R ShiK L Kalra
Oct 1, 1993·The Journal of Pathology·G CattorettiE Feudale
Aug 1, 1993·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·S Munakata, J B Hendricks

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Citations

Jan 1, 1996·Pathology Oncology Research : POR·Jamal BenfaresGyörgy Szekeres
Jun 26, 2001·Veterinary Dermatology·C LaprieM Delverdier
Mar 1, 1997·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·S R ShiC R Taylor

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