Effect of RNA synthesis inhibitors on insulin-induced protein synthesis by 3T3 cells.

The International Journal of Biochemistry
S Bardocz, J E Hesketh

Abstract

1. The increased protein synthesis of quiescent 3T3 cells in response to insulin was separated into three distinct phases based on their response to various inhibitors of RNA synthesis. 2. The first increase in protein synthesis was insensitive to the inhibitors used, and probably resulted from activation of existing protein synthesizing mechanism. 3. The second phase was sensitive to a varying extent to alpha-amanitin and 5,6-dichloro-1-beta-D-ribofuranosylbenzimidazole, implying the need for new mRNA synthesis as well as the production of new ribosomes indicated by its further sensitivity to low concentration (10 ng/ml) of Actinomycin D. 4. The final phase was insensitive to inhibitors of new ribosome formation, but still depended on new mRNA. alpha-difluoromethylornithine, an inhibitor of de novo polyamine synthesis, partly inhibited the insulin induced stimulation of protein synthesis.

References

Jan 1, 1978·Biochemical Pharmacology·P B Sehgal, I Tamm
Oct 1, 1970·Journal of Cellular Physiology·R P Perry, D E Kelley
Jul 29, 1980·Biochimica Et Biophysica Acta·L H Fahmy, D P Leader

❮ Previous
Next ❯

Citations

Jan 1, 1985·The International Journal of Biochemistry·E M Tyobeka, K L Manchester
Feb 1, 1992·The International Journal of Biochemistry·B AlmåsJ E Hesketh

❮ Previous
Next ❯

Related Concepts

Related Feeds

African Trypanosomiasis

African trypanosomiasis, also known as sleeping sickness, is an insect-borne parasitic disease of humans and other animals. It is caused by protozoa of the species Trypanosoma brucei and almost invariably progresses to death unless treated. Discover the latest research on African trypanosomiasis here.