Effect of Roux-en-Y gastric bypass on liver mitochondrial dynamics in a rat model of obesity

Physiological Reports
Jessica SacksJ P Kirwan

Abstract

Bariatric surgery provides significant and durable improvements in glycemic control and hepatic steatosis, but the underlying mechanisms that drive improvements in these metabolic parameters remain to be fully elucidated. Recently, alterations in mitochondrial morphology have shown a direct link to nutrient adaptations in obesity. Here, we evaluate the effects of Roux-en-Y gastric bypass (RYGB) surgery on markers of liver mitochondrial dynamics in a diet-induced obesity Sprague-Dawley (SD) rat model. Livers were harvested from adult male SD rats 90-days after either Sham or RYGB surgery and continuous high-fat feeding. We assessed expression of mitochondrial proteins involved in fusion, fission, mitochondrial autophagy (mitophagy) and biogenesis, as well as differences in citrate synthase activity and markers of oxidative stress. Gene expression for mitochondrial fusion genes, mitofusin 1 (Mfn1; P < 0.05), mitofusin 2 (Mfn2; P < 0.01), and optic atrophy 1 (OPA1; P < 0.05) increased following RYGB surgery. Biogenesis regulators, peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC1α; P < 0.01) and nuclear respiratory factor 1 (Nrf1; P < 0.05), also increased in the RYGB group, as well as mitophagy marker, BC...Continue Reading

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Citations

Aug 29, 2020·Journal of Cachexia, Sarcopenia and Muscle·Sanaz MehrabaniAmirhossein Sahebkar
Jul 22, 2019·International Journal of Obesity : Journal of the International Association for the Study of Obesity·S EzquerroA Rodríguez
Sep 16, 2018·Scientific Reports·P DongilC Sanz
Feb 27, 2020·Journal of the Endocrine Society·Margaret A StefaterNicholas Stylopoulos
Jan 12, 2021·American Journal of Physiology. Endocrinology and Metabolism·J David MosinskiJohn P Kirwan
Jan 15, 2020·Peptides·R Charlotte Moffett, Violetta Naughton
Aug 22, 2021·Trends in Molecular Medicine·Ciarán E FealyMatthijs K C Hesselink

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Methods Mentioned

BETA
gastric bypass
electrophoresis
Assay
electron microscopy

Software Mentioned

PrimerBank
GraphPad Prism
ImageJ

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