Effect of salt treatment on theanine biosynthesis in Camellia sinensis seedlings

Plant Physiology and Biochemistry : PPB
Wei-Wei DengXiang-Yang Hu

Abstract

Theanine synthetase (TS) is an enzyme involved in theanine biosynthesis in tea plants. Recent studies have revealed that theanine biosynthesis, derived from nitrogen metabolism in tea (Camellia sinensis L.) plants, could be influenced by salt treatment. We have characterized CsTS at the molecular and biochemical level. The expression pattern of CsTS protein was examined by western blot using a self-prepared polyclonal antibody with high specificity and sensitivity. The effect of salt treatment on the levels of theanine synthesis was investigated in this study. Levels of theanine and the total free amino acids were gradually increased in shoots, and reached the maximum on the 8th day after treatment (DAT). The immunoblotting analysis suggested the accumulation of CsTS protein had increased gently up to 8 DAT, and subsequently declined, both in roots and shoots, which is one of the main evidences that resulted in the variation of theanine concentration under salt treatment. Together, these data revealed that theanine synthesis takes place both in root and shoot and CsTS accumulation is positively affected by salt treatment.

References

Jul 25, 2006·Protein Expression and Purification·Li TianWei Dong Huang
Aug 15, 2009·Zeitschrift Für Naturforschung. C, a Journal of Biosciences·Wei-Wei DengHiroshi Ashihara
Nov 13, 2010·Plant & Cell Physiology·Wei-Wei Deng, Hiroshi Ashihara

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Citations

Nov 14, 2015·Plant Cell Reports·Mainaak MukhopadhyayPradeep K Chand
Jan 13, 2019·International Journal of Molecular Sciences·Jiaojiao ZhuYuhua Wang
Jan 21, 2020·Food Research International·Chaozhen ZengZhonghua Liu

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