Effect of salvianolic acid B on proliferation and apoptosis of human nasal polyps fibroblasts

Lin chuang er bi yan hou tou jing wai ke za zhi = Journal of clinical otorhinolaryngology, head, and neck surgery
D LuoZ F Sun

Abstract

Objective:The aim of this study is to discuss the effects and mechanism of salvianolic acid B (SAB) on the proliferation and apoptosis of human nasal polyps fibroblasts (NPFs). Method:The NPFs were treated with SAB at different concentrations (5, 10, 20, 40 μmol/L). CCK-8 assay was used to determine the inhibition of SAB on the proliferation of NPFs and determined the optimum concentration for the following experiments. The NPFs were divided into four groups: a) control group: the cells were cultured in DMEM without TGF-β1 or salvianolic acid B; b) the TGF-β1 group: the cells were cultured in DMEM containing 5 ng/ml TGF-β1; c) the SAB group: the cells were cultured in DMEM containing SAB at the optimum concentration by CCK-8 assay; d) the TGF-β1+SAB treatment groups: the cells were cultured in DMEM containing 5 ng/ml TGF-β1 and SAB at the optimum concentration by CCK-8 assay. MTT assay was used to determine the proliferation of NPFs in four groups. The apoptosis rate was analyzed by Annexin-V FITC/PI double staining. The activities of Caspase-3 were detected with spectrophotometric method. Apoptosis related proteins Bcl-2 and Bax were measured by western blot. Result:CCK-8 assay showed that SAB could inhibite NPFs proliferation...Continue Reading

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