Effect of sanguinarine from the leaves of Macleaya cordata against Ichthyophthirius multifiliis in grass carp (Ctenopharyngodon idella).

Parasitology Research
Jia-Yun YaoWen-Lin Yin

Abstract

The ciliate Ichthyophthirius multifiliis is one of the most pathogenic parasites of fish maintained in captivity. In this study, effects of crude extracts, fractions, and compounds from the leaves of Macleaya cordata against I. multifiliis were investigated under in vitro conditions by bioactivity-guided isolation method. The dried ethanol extract of M. cordata was extracted successively in a separating funnel with petroleum ether, ethyl acetate, chloroform and n-butanol. Among them, only the chloroform extract showed promising activity and therefore, was subjected to further separation and purification using various chromatographic techniques. Four compounds were isolated from chloroform extract, but only one compound showed potent activity. The structure of the active compound was elucidated as sanguinarine by hydrogen, carbon-13 nuclear magnetic resonance spectrum and electron ionization mass spectrometry. In vitro antiparasitic efficacy tests exhibited that sanguinarine was 100% effective against I. multifiliis at a concentration of 0.7 mg l(-1), with LC(50) and LC(90) values of 0.437 and 0.853 mg l(-1) after 4 h of exposure. In vivo antiparasitic efficacy tests showed that the number of I. multifiliis on the gills in the t...Continue Reading

References

Feb 1, 1993·Statistics in Medicine·J W DraneC Stoskopf
Jan 1, 1996·Journal of Enzyme Inhibition·J DrsataD Walterová
Aug 1, 1998·Journal of Ethnopharmacology·L TonaA J Vlietinck
Aug 5, 1999·Journal of Ethnopharmacology·V Navarro, G Delgado
May 29, 2000·Parasitology Today·M L Willcox, G Bodeker
May 5, 2001·Annual Review of Plant Physiology and Plant Molecular Biology·Peter J Facchini
Jul 9, 2002·Phytochemical Analysis : PCA·R M Perez GutierrezF J Martinez-Martinez
Dec 25, 2002·Biological & Pharmaceutical Bulletin·Tadaaki SatouTamotsu Nikaido
Jul 26, 2003·Journal of Natural Products·David J NewmanKenneth M Snader
Nov 25, 2003·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Pavel KosinaVilím Simánek
Jul 14, 2007·Journal of Ethnopharmacology·Fernando CalzadaAmparo Tapia-Contreras
Jan 19, 2008·Journal of Aquatic Animal Health·Franz Lahnsteiner, Thomas Weismann
Feb 18, 2009·Parasitology Research·David L Straus, Thomas Meinelt
Mar 24, 2009·Diseases of Aquatic Organisms·David L StrausTheodore G Clark

❮ Previous
Next ❯

Citations

Aug 27, 2014·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Xuelong YuXingyun Chai
Jan 27, 2015·Journal of Veterinary Pharmacology and Therapeutics·G M R ValladãoF Pilarski
May 4, 2013·International Journal for Parasitology. Drugs and Drug Resistance·Si-Ming Zhang, Kristen A Coultas
Sep 1, 2018·Journal of Fish Diseases·Seyed Jalil AlaviniaHosseinali Ebrahimzadeh Mousavi
Jul 3, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Sharna-Kay Daley, Geoffrey A Cordell
Jan 22, 2015·Journal of Agricultural and Food Chemistry·Jing-Han LiangDe-Jie Lin
Dec 5, 2020·Biochemical and Biophysical Research Communications·Hao ChiZhaobing Gao

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antiparasitics

Antiparasitics are medications which are indicated for the treatment of parasitic diseases. Discover the latest research on antiparasitics here.