Effect of SATB1 silencing on the proliferation, invasion and apoptosis of TE-1 esophageal cancer cells

Oncology Letters
Bo HuangHongli Zhou

Abstract

The aim of the present study was to investigate the effect of special AT-rich sequence-binding protein-1 (SATB1)-targeted small interfering RNA (siRNA) on the proliferation, invasion and apoptosis of TE-1 human esophageal cancer cells. SATB1 has been correlated with the metastasis and poor prognosis of colon and breast cancer, but the role of SATB1 in esophageal cancer remains unknown. Therefore, the present study constructed and transfected SATB1-siRNA into TE-1 cells in order to knockdown the expression of the SATB1 gene. Western blot analysis, a cell counting kit, transwell chamber assays and flow cytometry were used to assess the effect of SATB1-siRNA on the proliferation, invasion and apoptosis of cells. The results demonstrated that the expression of the SATB1 gene was efficiently knocked down by SATB1-siRNA, and that SATB1-siRNA inhibited the proliferation, invasion and apoptosis of TE-1 cells. Therefore, it was concluded that the SATB1 gene is important in the pathogenesis of human esophageal cancer, and may present a novel therapeutic target for esophageal cancer.

References

Jan 1, 1985·Virchows Archiv. B, Cell Pathology Including Molecular Pathology·F G GudatJ P Obrecht
Apr 15, 2003·Nature Genetics·Shutao CaiTerumi Kohwi-Shigematsu
Mar 14, 2008·Nature·Hye-Jung HanTerumi Kohwi-Shigematsu
Jun 19, 2008·Cancer Epidemiology, Biomarkers & Prevention : a Publication of the American Association for Cancer Research, Cosponsored by the American Society of Preventive Oncology·Shu-Chun ChuangPaul Brennan
Jun 4, 2010·European Journal of Gastroenterology & Hepatology·Shutao ZhengXiaomei Lu
Apr 18, 2012·Chinese Journal of Cancer·Han-Ze ZhangHong-Bing Shen
Jul 10, 2012·Seminars in Cancer Biology·Terumi Kohwi-ShigematsuYoshinori Kohwi
Dec 20, 2012·PloS One·Ellen OrdinarioTerumi Kohwi-Shigematsu
Jan 3, 2013·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Chang J KimNeung H Park
Feb 23, 2013·International Immunopharmacology·Jakub GrzankaPiotr Witkowski
Apr 25, 2013·World Journal of Gastroenterology : WJG·Xue-Feng FangYing Yuan
Feb 6, 2014·Folia Histochemica Et Cytobiologica·Christopher KobierzyckiPiotr Dziegiel
Apr 15, 2014·International Journal of Cancer. Journal International Du Cancer·Anja FrömbergAchim Aigner
Oct 3, 2015·Carbohydrate Polymers·Bhawna SoniBarakat Mahmoud

❮ Previous
Next ❯

Methods Mentioned

BETA
surgical resection
flow cytometry
transfection

Software Mentioned

Quantity One
SPSS

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Apoptosis in Cancer

Apoptosis is an important mechanism in cancer. By evading apoptosis, tumors can continue to grow without regulation and metastasize systemically. Many therapies are evaluating the use of pro-apoptotic activation to eliminate cancer growth. Here is the latest research on apoptosis in cancer.

Breast Invasive Carcinoma (Keystone)

Invasive breast cancers indicate a spread into breast tissues and lymph nodes. Here are the latest discoveries pertaining to breast invasive carcinomas.

Breast Invasive Carcinoma

Invasive breast cancers indicate a spread into breast tissues and lymph nodes. Here are the latest discoveries pertaining to breast invasive carcinomas.