Abstract
The aim of the present study was to investigate the effect of special AT-rich sequence-binding protein-1 (SATB1)-targeted small interfering RNA (siRNA) on the proliferation, invasion and apoptosis of TE-1 human esophageal cancer cells. SATB1 has been correlated with the metastasis and poor prognosis of colon and breast cancer, but the role of SATB1 in esophageal cancer remains unknown. Therefore, the present study constructed and transfected SATB1-siRNA into TE-1 cells in order to knockdown the expression of the SATB1 gene. Western blot analysis, a cell counting kit, transwell chamber assays and flow cytometry were used to assess the effect of SATB1-siRNA on the proliferation, invasion and apoptosis of cells. The results demonstrated that the expression of the SATB1 gene was efficiently knocked down by SATB1-siRNA, and that SATB1-siRNA inhibited the proliferation, invasion and apoptosis of TE-1 cells. Therefore, it was concluded that the SATB1 gene is important in the pathogenesis of human esophageal cancer, and may present a novel therapeutic target for esophageal cancer.
References
Jan 1, 1985·Virchows Archiv. B, Cell Pathology Including Molecular Pathology·F G GudatJ P Obrecht
Apr 15, 2003·Nature Genetics·Shutao CaiTerumi Kohwi-Shigematsu
Apr 25, 2006·Molecular Cell·P Pavan KumarSanjeev Galande
Mar 14, 2008·Nature·Hye-Jung HanTerumi Kohwi-Shigematsu
Jun 19, 2008·Cancer Epidemiology, Biomarkers & Prevention : a Publication of the American Association for Cancer Research, Cosponsored by the American Society of Preventive Oncology·Shu-Chun ChuangPaul Brennan
Dec 17, 2008·Women's Health·Jie Zheng
Jun 4, 2010·European Journal of Gastroenterology & Hepatology·Shutao ZhengXiaomei Lu
Apr 18, 2012·Chinese Journal of Cancer·Han-Ze ZhangHong-Bing Shen
Jul 10, 2012·Seminars in Cancer Biology·Terumi Kohwi-ShigematsuYoshinori Kohwi
Dec 20, 2012·PloS One·Ellen OrdinarioTerumi Kohwi-Shigematsu
Jan 3, 2013·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Chang J KimNeung H Park
Feb 6, 2013·Cancer Cell International·Bo HuangZhiliang Liu
Feb 23, 2013·International Immunopharmacology·Jakub GrzankaPiotr Witkowski
Apr 25, 2013·World Journal of Gastroenterology : WJG·Xue-Feng FangYing Yuan
Oct 15, 2013·Histopathology·Sam Al-SohailyJanindra Warusavitarne
Feb 6, 2014·Folia Histochemica Et Cytobiologica·Christopher KobierzyckiPiotr Dziegiel
Mar 29, 2014·PloS One·Zhao PengQiang Tong
Apr 15, 2014·International Journal of Cancer. Journal International Du Cancer·Anja FrömbergAchim Aigner
Apr 22, 2014·Blood·Yang WangPing Tu
Oct 3, 2015·Carbohydrate Polymers·Bhawna SoniBarakat Mahmoud
Nov 12, 2016·BMC Cancer·Lei ZhouDanna Wang
Citations
Nov 19, 2019·Disease Markers·Marta SmolińskaAnna Klimaszewska-Wiśniewska
Feb 23, 2018·Cell Death & Disease·Xin PanXiaoxin Ma
Dec 18, 2018·Life Sciences·Hong-Yu XuYou Lu