PMID: 2494147Mar 1, 1989Paper

Effect of scavengers of active oxygen species and pretreatment with acetyl-salicylic acid on the injury to cultured endothelial cells by thrombin-stimulated platelets

In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association
T LarsenL Jørgensen

Abstract

Thrombin-stimulated human platelets adhere to and injure cultured human endothelial cells. We hypothesize that generation of active oxygen species by the stimulated platelets are involved in the injury. To confirm this, catalase [final concentration (8.25 micrograms/ml)], superoxide dismutase (SOD) (10 micrograms/ml), of D-mannitol (9 mg/ml) were added to the cell culture medium before the experiments. Platelet suspension (200,000/microliters) and thrombin (4 U/ml) were added and the culture dishes shaken for 15 min at room temperature. In separate experiments the endothelial cells were pretreated with acetylsalicylic acid (0.05, 0.1, or 0.5 mM) to test whether the arachidonic acid metabolism of the endothelial cells is involved in the injury process. In preliminary experiments we were able to confirm that platelets, when stimulated by thrombin, produce chemiluminescence which was suppressed by mannitol but not by catalase or SOD. The degree of injury to cultured endothelial cells by thrombin-stimulated platelets, as measured by release of 51Cr from prelabeled endothelial cells, was reduced significantly with the presence of mannitol, but only moderately when catalase or SOD had been added. Morphometric quantification based on ...Continue Reading

References

Feb 1, 1977·The Journal of Clinical Investigation·I M GoldsteinH B Kaplan
Jul 1, 1978·Annals of Internal Medicine·J M McCord, I Fridovich
Mar 1, 1979·The Journal of Clinical Investigation·E A Jaffe, B B Weksler
Apr 1, 1979·Clinical Endocrinology·J A WassA V Schally
Jan 1, 1978·Advances in Enzyme Regulation·J A MontgomeryL L Bennett
Mar 1, 1978·The Journal of Clinical Investigation·E L MillsP G Quie
Jan 13, 1977·Nature·F A KuehlC G Van Arman
Mar 8, 1976·Biochemical and Biophysical Research Communications·R C Allen, L D Loose
Nov 1, 1986·The American Journal of Physiology·N SuttorpL Roka
Mar 1, 1985·Stroke; a Journal of Cerebral Circulation·T FujimotoH Yamazaki
Feb 28, 1985·Biochemical and Biophysical Research Communications·M F TsanC L Rosano
Nov 1, 1985·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·S SolbergL Jørgensen
Feb 1, 1972·British Journal of Haematology·J F MustardM A Packham
Nov 1, 1980·The Journal of Clinical Investigation·A J MarcusM J Broekman
Oct 29, 1982·Biochemical and Biophysical Research Communications·A R MorrisonW A Brown
Oct 1, 1962·The Journal of Pathology and Bacteriology·A HUGHES, R S TONKS

❮ Previous
Next ❯

Citations

Nov 1, 1985·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·S SolbergL Jørgensen
Jun 1, 1991·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·R H Goodwin
Mar 1, 1997·In Vitro Cellular & Developmental Biology. Animal·I B HellandL Jørgensen
Jan 1, 1992·Free Radical Research Communications·S KitagawaH Sakurai
Jul 15, 2006·Journal of Thrombosis and Haemostasis : JTH·L Jørgensen

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.