Effect of secretin on preadipocyte, differentiating and mature adipocyte functions

International Journal of Obesity : Journal of the International Association for the Study of Obesity
P MiegueuD H St-Pierre

Abstract

Several gastrointestinal peptides are now recognized to have target functions beyond the intestinal wall, including effects on adipocytes. Secretin (SEC), one of the first identified, has not been evaluated in this context. Using cultured 3T3-L1 preadipocytes, adipocytes and primary rat adipocytes we evaluated the effect of SEC on cell proliferation, mitochondrial activity, differentiation, triglyceride (TG) synthesis, lipolysis as well expression of the SEC receptor (SCTR) in rodent and human adipose tissues. In preadipocytes, SEC significantly increased mitochondrial activity (115%; P<0.01), thymidine incorporation (149.7%; P<0.05) and C/EBPβ expression (123.4%; P<0.05). During standard differentiation, SCTR mRNA increased up to a maximum of ninefold (P<0.001). In human adipose tissue, SCTR correlated with body mass index and plasma insulin, and SCTR mRNA expression was also detected in rat adipose tissues. SEC supplementation during differentiation enhanced TG accumulation (+138%; P<0.01). In mature adipocytes, SEC increased fatty acid (FA) uptake (186%; P<0.01), adiponectin and monocyte chemotactic protein-1 secretion (+142% and +149%, respectively; P<0.05) and mRNA expression of PPARγ (+206%; P<0.01), FABP4 (+164%; P<0.001...Continue Reading

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Citations

Nov 26, 2013·Journal of Lipid Research·Revathi Sekar, Billy K C Chow
Dec 19, 2012·General and Comparative Endocrinology·Revathi Sekar, Billy K C Chow
Apr 27, 2016·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Nan WuGianfranco Alpini
Mar 9, 2018·The Journal of Experimental Biology·Katharina BraunMartin Klingenspor
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Apr 29, 2014·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Revathi Sekar, Billy K C Chow
Apr 10, 2020·Experimental Physiology·Katharina SchnablMartin Klingenspor
Nov 7, 2015·Applied Biochemistry and Biotechnology·Erkan MoziogluMuslum Akgoz
Oct 4, 2020·Biochemical and Biophysical Research Communications·Satoshi FukuharaOsamu Nureki
May 1, 2021·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Lixian ChenGianfranco Alpini
Apr 10, 2018·Journal of Agricultural and Food Chemistry·Chen-Yuan ChiuMeng-Tsan Chiang

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