PMID: 9434117Jan 20, 1998Paper

Effect of selective cysteine --> alanine replacements on the catalytic functions of lysine: N6-hydroxylase

Biochimica Et Biophysica Acta
L Marrone, T Viswanatha

Abstract

Recombinant lysine: N6-hydroxylase, rIucD, catalyzes the conversion of L-lysine to its N6-hydroxy derivative. Re-examination of the nucleotide sequence of iucD, the gene encoding for the enzyme, has revealed a few discrepancies in the data documented in literature and the corrected version is presented. The revised nucleotide sequence predicts the presence of five cysteine residues in the primary structure of IucD. Two of these residues, cysteine 51 and cysteine 158 are alkylatable by iodoacetate in the native conformation of the protein resulting in a loss of monooxygenase activity while their replacement with alanine has no such adverse effect. Site directed mutagenesis studies have enabled an assessment of the reactivity of these cysteine residue(s) towards thiol modifying agents.

References

Apr 1, 1979·Analytical Biochemistry·P W RiddlesB Zerner
Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Nov 24, 1979·Nucleic Acids Research·H C Birnboim, J Doly
Jul 11, 1979·Biochimica Et Biophysica Acta·M A ParniakT Viswanatha
Jan 1, 1978·Methods in Enzymology·A G HildebrandtG Heinemeyer
Mar 24, 1989·Biochimica Et Biophysica Acta·C J GohT Viswanatha
Feb 1, 1986·Journal of Bacteriology·V de LorenzoJ B Neilands
Dec 1, 1971·Analytical Biochemistry·G TomlinsonT Viswanatha
Nov 22, 1994·Proceedings of the National Academy of Sciences of the United States of America·G Siuzdak
Nov 10, 1993·Biochimica Et Biophysica Acta·A M ThariathT Viswanatha
May 1, 1993·European Journal of Biochemistry·P MacherouxH Diekmann
Jul 1, 1996·Archives of Biochemistry and Biophysics·A J TrimboliM J Barber
Oct 1, 1958·Biochimica Et Biophysica Acta·V MASSEY
May 1, 1959·Archives of Biochemistry and Biophysics·G L ELLMAN
Nov 1, 1963·The Journal of Pediatrics·F HUIJINGG LOSEKOOT
Sep 1, 1963·Biochemistry·H I HADLER, M J ERWIN
Jul 28, 2012·Chemistry : a European Journal·Kara L CubbageKevin I Booker-Milburn
Jan 1, 1972·Methods in Enzymology·F R Gurd

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.