Effect of selective inhibition of aquaporin 1 on chemotherapy sensitivity of J82 human bladder cancer cells

Oncology Letters
Xuefeng ZhangBenkang Shi

Abstract

The occurrence of resistance to mitomycin C (MMC) often limits its clinical effectiveness. Combination therapy thus is employed to overcome this treatment resistance. The present study aimed to establish a novel J82 bladder cancer cell line so as to study the effect of inhibition of aquaporin 1 (AQP-1) on chemotherapy sensitivity of J82 bladder cancer cells. A novel J82 bladder cancer cell line whose expression of AQP-1 is inhibited was established through transfection of J82 cells with newly constructed recombinant plasmid. The resulting cell line was designated J82-short hairpin (sh)AQP1 and was subjected to further analyses together with J82 cell line. Reverse transcription-polymerase chain reaction was used to quantify the expression of AQP-1mRNA in the cells; cell viability was analyzed with MTT assay and apoptosis was measured by flow cytometry. The expression of cell proliferation and cell apoptosis-associated proteins, proliferating cell nuclear antigen (PCNA), B cell lymphoma 2 (Bcl-2), Bcl-2 associated X protein (Bax) and caspase-3, were detected by Western blot. A statistically significant decrease in the transcription and expression of AQP1 was observed in the J82-shAQP1 cells as compared with J82 cells. J82-shAQP1 ...Continue Reading

References

Dec 1, 1985·Cancer Treatment Reviews·L Lenaz
Oct 30, 2001·International Journal of Radiation Biology·T PauneskuG E Woloschak
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Aug 25, 2004·Cancer Cell·Colin P N DinneyBogdan Czerniak
Oct 21, 2006·Cell Death and Differentiation·M LamkanfiP Vandenabeele
Mar 4, 2008·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·A S VerkmanMarios C Papadopoulos
Mar 29, 2008·Pharmaceutical Research·Zancong ShenJessie L-S Au
Jun 23, 2009·Peptides·Yogendra B ShresthaSilvia Giraudo
Aug 21, 2012·Asian Pacific Journal of Cancer Prevention : APJCP·Yong-Hua ShiSuzuke Lalai
Mar 2, 2013·The Journal of Clinical Endocrinology and Metabolism·Li-Bo ZouJian-Zhong Sheng
May 18, 2013·Asian Pacific Journal of Cancer Prevention : APJCP·Yong-Hua ShiLalai Suzuke
Sep 10, 2013·Journal of Surgical Oncology·Nicolai El HindyI Erol Sandalcioglu
Feb 5, 2014·Otolaryngology--head and Neck Surgery : Official Journal of American Academy of Otolaryngology-Head and Neck Surgery·Marietta TanPatrick K Ha
Mar 13, 2014·Nature Reviews. Urology·Homayoun ZargarPeter Black
May 13, 2014·International Journal of Molecular Sciences·Minyong KangHyeon Hoe Kim
Aug 16, 2014·Asian Pacific Journal of Cancer Prevention : APJCP·Yong-Hua ShiHeng Chang
May 20, 2015·Asian Pacific Journal of Cancer Prevention : APJCP·Jie LiuDe-Gang Ding
Jul 3, 2016·European Urology·Sebastien AntoniFreddie Bray

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