Effect of selective phosphodiesterase inhibitors on response of ovine pulmonary arteries to prostaglandin E2

Journal of Applied Physiology
Y GaoJ U Raj

Abstract

Several adenosine 3',5'-cyclic monophosphate (cAMP)-hydrolyzing phosphodiesterase isozymes are present in the pulmonary vasculature. The present study was designed to determine the effect of selective inhibitors of phosphodiesterase subtypes on prostaglandin E2 (PGE2)-induced relaxation of isolated fourth-generation pulmonary arteries of newborn lambs. PGE2 and forskolin caused pulmonary arteries to relax and induced an increase in the intracellular cAMP content in the vessels. The relaxation and change in cAMP content were augmented by milrinone and rolipram, inhibitors of phosphodiesterase type 3 (PDE3) and type 4 (PDE4), respectively. The augmentation in relaxation and the increase in cAMP content caused by milrinone plus rolipram was greater than the sum of the responses caused by either of the inhibitors alone. 8-Methoxymethyl-1-methyl-3-(2-methylpropyl)xanthine, an inhibitor of phosphodiesterase type 1, had no effect on relaxation and change in cAMP induced by PGE2 and forskolin. Acetylcholine alone had no effect on cAMP content in the vessels but augmented the relaxation and the increase in cAMP induced by PGE2 and forskolin in arteries with endothelium. This effect was not observed in arteries without endothelium or in ...Continue Reading

References

Dec 1, 1992·The Journal of Clinical Investigation·P W ShaulR R Magness
Aug 1, 1992·Journal of Smooth Muscle Research = Nihon Heikatsukin Gakkai Kikanshi·T MoritaY Tashima
Jan 10, 1991·European Journal of Pharmacology·D H MauriceR J Haslam
Apr 1, 1990·Trends in Pharmacological Sciences·J A Beavo, D H Reifsnyder
Jan 1, 1990·Annual Review of Pharmacology and Toxicology·L J Ignarro
Jan 1, 1988·Methods in Enzymology·J N Wells, J R Miller
Mar 22, 1988·European Journal of Pharmacology·G C GraceG J Dusting
May 18, 1984·European Journal of Pharmacology·T M Lincoln, V Fisher-Simpson
Jan 1, 1995·Annual Review of Physiology·J R FinemanM A Heymann
Jan 9, 1996·Proceedings of the National Academy of Sciences of the United States of America·J Jurevicius, R Fischmeister
Jan 1, 1996·Annual Review of Pharmacology and Toxicology·J B Polson, S J Strada

❮ Previous
Next ❯

Citations

Dec 6, 2008·Pediatric Critical Care Medicine : a Journal of the Society of Critical Care Medicine and the World Federation of Pediatric Intensive and Critical Care Societies·Satyan LakshminrusimhaRobin H Steinhorn
Oct 21, 2010·Physiological Reviews·Yuansheng Gao, J Usha Raj
Dec 22, 2009·Pflügers Archiv : European journal of physiology·Yuansheng Gao
Aug 27, 2009·Pediatric Research·Bernadette ChenRobin H Steinhorn
Oct 25, 2011·Pflügers Archiv : European journal of physiology·Lei YingYuansheng Gao
Mar 11, 2003·Reproductive Biomedicine Online·Philip S LaPoltJean You-hsin Chen

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.