Effect of semen collection method (artificial vagina vs. electroejaculation), extender and centrifugation on post-thaw sperm quality of Blanca-Celtibérica buck ejaculates

Animal Reproduction Science
Pilar Jiménez-RabadánA J Soler

Abstract

The aim of this study was to evaluate the effect of semen collection method (artificial vagina compared to electroejaculation), season in which the semen was collected (breeding season compared to non-breeding season), freezing extender (Biladyl(®), Andromed(®) and skim milk based extender) and pre-treatment procedure (washing compared to non-washing) on post-thaw semen quality in buck. Ejaculates from seven bucks of the Blanca-Celtibérica breed were collected by artificial vagina and electroejaculation during the breeding (July to December) and non-breeding season (January to June). Samples were split in two aliquots and one of them was washed. Three freezing extenders were evaluated on washing and non-washing sperm samples. Ejaculates collected by artificial vagina had a greater sperm quality after thawing, with greater values (P≤0.05) for SM (sperm motility), NAR (acrosome intact), YO-PRO-1-/PI- (intact spermatozoa), and Mitotracker+/YO-PRO-1- (spermatozoa with active mitochondria) and lower % DFI (DNA fragmentation index). Thawed sperm samples which were collected during the breeding season had greater values (P≤0.05) for NAR, intact spermatozoa and spermatozoa with active mitochondria, than those semen samples obtained dur...Continue Reading

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Citations

Sep 26, 2013·Journal of Zoo and Wildlife Medicine : Official Publication of the American Association of Zoo Veterinarians·Marisa BezjianKatherine A Beltaire
Dec 4, 2012·International Journal of Molecular Sciences·Clara González-MarínRosa Roy
Feb 27, 2016·Animal Reproduction Science·P Jiménez-RabadánJ J Garde
Feb 19, 2014·Journal of Dairy Science·Lavanya GoodlaAnders Johannisson
Oct 23, 2012·Animal Reproduction Science·P Jiménez-RabadánA J Soler
May 24, 2018·Reproduction : the Official Journal of the Society for the Study of Fertility·F Buchelly ImbachíA Cesari
Oct 18, 2018·Biology of Reproduction·Laila A IbrahimJohn J Bromfield
Nov 28, 2016·Journal of Dairy Science·Edel M MurphySean Fair
Oct 24, 2019·BMC Veterinary Research·Kirsten HahnAxel Wehrend
Jul 9, 2020·Frontiers in Veterinary Science·Arumugam KumaresanJane M Morrell
Nov 4, 2020·Animals : an Open Access Journal From MDPI·Sara El KadiliMouad Chentouf
May 6, 2019·Theriogenology·T NadriA Mohammadi Sangcheshmeh
Jul 3, 2021·Animals : an Open Access Journal From MDPI·Luciana Diniz RolaJosé Maurício Barbanti Duarte
Aug 7, 2021·Biology·Aitor Fernandez-NovoSonia Salome Perez-Garnelo

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