Abstract
Artificial insemination with doses containing low-sperm numbers has been utilized to optimize the use of elite bulls. Hen egg yolk is widely used as a cryoprotective agent in semen freezing extender protecting the spermatozoa. Its action is due to the presence of low-density lipoproteins (LDL) in the hen egg yolk. The objectives of the present study were to evaluate the effects of the semen dilution to low-sperm number/dose on sperm motility and integrity of sperm plasma membrane in the cryopreservation process, using two commercial extenders (Triladyl, Bioxcell and LDL extender prepared in our laboratory, 97% purity. Fifteen ejaculates were collected from five fertile crossbred bulls (Bos taurusxBos indicus). After collection, sperm motility was examined by Computer-Assisted Semen Analysis (Hamilton Thorne), morphological sperm characteristics were evaluated by differential interference microscopy and the integrity of plasma membranes was determined using the hypo-osmotic swelling test. The semen was subsequently divided into three aliquots and diluted with the three extenders into 120 x 10(6), 60 x 10(6) and 20 x 10(6)sperm/mL, corresponding to 30 x 10(6), 15 x 10(6) and 5 x10(6) sperm/dose, respectively. This study revealed ...Continue Reading
References
Sep 23, 1997·Cryobiology·H WoeldersB Engel
Mar 29, 2000·Theriogenology·W M Maxwell, L A Johnson
Jul 27, 2001·Theriogenology·D L GarnerC H Allen
May 31, 2002·Theriogenology·M MoussaM Anton
Oct 2, 2003·Theriogenology·Leonardo F C BritoJohn P Kastelic
Feb 6, 2004·Theriogenology·Lamia AmiratMarc Anton
Mar 24, 2004·Theriogenology·Magnus AnderssonMerja Dahlbom
Jun 1, 2005·Animal Reproduction Science·N S PrathalingamP F Watson
Aug 1, 1994·Theriogenology·J R Correa, P M Zavos
Jul 27, 2006·Molecular Reproduction and Development·Annick Bergeron, Puttaswamy Manjunath
Aug 21, 2007·Theriogenology·J BallesterH Rodriguez-Martinez
Citations
Mar 23, 2011·Asian Journal of Andrology·Qiao-Xiang DongCatherine A Vandevoort
Nov 30, 2010·Asian Journal of Andrology·Oscar Vera-MunozDaniel Tainturier
Dec 1, 2012·Zygote : the Biology of Gametes and Early Embryos·Peng WangGong-She Yang
Sep 23, 2014·Animal Reproduction Science·Alessia GloriaAugusto Carluccio
Dec 4, 2014·Animal Reproduction Science·N IaffaldanoA Manchisi
Jan 15, 2013·Reproduction, Fertility, and Development·M Alvarez-RodríguezP de Paz
Jan 5, 2011·Cryobiology·Jian-Hong HuXin Li
Oct 22, 2010·Animal Reproduction Science·L Amirat-BriandD Tainturier
May 25, 2010·Theriogenology·S Pérez-CerezalesM P Herráez
Feb 1, 2015·Veterinary World·D V ChaudhariJ A Patel
Aug 12, 2016·Animal Reproduction Science·S S LayekJ E Parks
Feb 7, 2018·Reproduction in Domestic Animals = Zuchthygiene·A PinyopumminP Wattananit
Jul 3, 2015·PloS One·Vivek Phani VarmaSandeep Goel
Aug 19, 2020·Systems Biology in Reproductive Medicine·Alicja KowalczykEwa Czerniawska-Piątkowska
Nov 23, 2019·Animals : an Open Access Journal From MDPI·Ayman Abdel-Aziz SwelumHany Abdalla
Jan 8, 2021·Cryobiology·Rommy DíazJorge G Farías
Nov 16, 2020·Theriogenology·Theresa FichtnerChristine Wrenzycki