Effect of semen dilution to low-sperm number per dose on motility and functionality of cryopreserved bovine spermatozoa using low-density lipoproteins (LDL) extender: comparison to Triladyl and Bioxcell

Theriogenology
Oscar Vera-MunozDaniel Tainturier

Abstract

Artificial insemination with doses containing low-sperm numbers has been utilized to optimize the use of elite bulls. Hen egg yolk is widely used as a cryoprotective agent in semen freezing extender protecting the spermatozoa. Its action is due to the presence of low-density lipoproteins (LDL) in the hen egg yolk. The objectives of the present study were to evaluate the effects of the semen dilution to low-sperm number/dose on sperm motility and integrity of sperm plasma membrane in the cryopreservation process, using two commercial extenders (Triladyl, Bioxcell and LDL extender prepared in our laboratory, 97% purity. Fifteen ejaculates were collected from five fertile crossbred bulls (Bos taurusxBos indicus). After collection, sperm motility was examined by Computer-Assisted Semen Analysis (Hamilton Thorne), morphological sperm characteristics were evaluated by differential interference microscopy and the integrity of plasma membranes was determined using the hypo-osmotic swelling test. The semen was subsequently divided into three aliquots and diluted with the three extenders into 120 x 10(6), 60 x 10(6) and 20 x 10(6)sperm/mL, corresponding to 30 x 10(6), 15 x 10(6) and 5 x10(6) sperm/dose, respectively. This study revealed ...Continue Reading

References

Mar 29, 2000·Theriogenology·W M Maxwell, L A Johnson
Jul 27, 2001·Theriogenology·D L GarnerC H Allen
Jul 27, 2006·Molecular Reproduction and Development·Annick Bergeron, Puttaswamy Manjunath
Aug 21, 2007·Theriogenology·J BallesterH Rodriguez-Martinez

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Citations

Dec 1, 2012·Zygote : the Biology of Gametes and Early Embryos·Peng WangGong-She Yang
Sep 23, 2014·Animal Reproduction Science·Alessia GloriaAugusto Carluccio
Aug 12, 2016·Animal Reproduction Science·S S LayekJ E Parks
Feb 7, 2018·Reproduction in Domestic Animals = Zuchthygiene·A PinyopumminP Wattananit
Jul 3, 2015·PloS One·Vivek Phani VarmaSandeep Goel
Aug 19, 2020·Systems Biology in Reproductive Medicine·Alicja KowalczykEwa Czerniawska-Piątkowska
Nov 23, 2019·Animals : an Open Access Journal From MDPI·Ayman Abdel-Aziz SwelumHany Abdalla

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