Effect of sensory denervation on the structure and physiologic responsiveness of rabbit lacrimal gland

Cornea
M A MenerayRoger W Beuerman

Abstract

This work was conducted to determine the effects of unilateral trigeminal ganglion ablation on lacrimal gland structure and secretory activity. Adult male New Zealand rabbits underwent unilateral thermocoagulation of the ophthalmic division of the trigeminal ganglion. Sensory denervation was affirmed by anatomic inspection of the lesion and transmission electron microscopy (TEM) of the lacrimal gland innervation. Eight to 10 days after the procedure, the intraorbital lacrimal glands were removed from both sides. To compare the physiologic competence of the intact and denervated glands, freshly isolated gland fragments from the paired intact and denervated glands were stimulated with carbachol (100 microM), isoproterenol (10 microM), phorbol-12,13-dibutyrate (PDBu, 10 microM), forskolin (40 microM), or vehicle. Total secreted protein was measured at 30 or 60 min after the establishment of baseline values. Intact and denervated glands also were examined by light and TEM, and the morphologic appearance of the acinar structures as well as the appearance of nerves innervating the gland after denervation were assessed. Similar experiments were conducted with animals that underwent unilateral superior cervical ganglionectomy. Tissues ...Continue Reading

Citations

Sep 28, 2007·Investigative Ophthalmology & Visual Science·Hiroshi ToshidaAkira Murakami
Feb 17, 2009·Investigative Ophthalmology & Visual Science·Hiroshi ToshidaAkira Murakami
Sep 9, 1999·In Vitro Cellular & Developmental Biology. Animal·D H NguyenG Sun
Apr 11, 2006·Current Eye Research·Doan H NguyenHiroshi Toshida
Apr 21, 2009·Progress in Retinal and Eye Research·Darlene A Dartt
Dec 23, 2008·Journal of Cataract and Refractive Surgery·Miltiadis PapathanassiouIoannis Vergados
Jun 29, 2004·Progress in Retinal and Eye Research·Michael E Johnson, Paul J Murphy
Jul 24, 2015·Cornea·Stephanie M Cox, Jason J Nichols
Apr 3, 2012·Peptides·Cecilia Dall'aglioCristiano Boiti
Dec 26, 2001·The Journal of Pharmacology and Experimental Therapeutics·J PintorB Yerxa
Feb 23, 2020·The American Journal of Pathology·Kai JinKazuo Tsubota

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