Effect of serum subfractions from peritoneal dialysis patients on Hep-G2 cell apolipoprotein A-I and B metabolism

Kidney International
G M ShahM A Kirschenbaum

Abstract

We previously showed that uremic serum subfractions isolated from hemodialysis (HD) patients inhibited the production of apolipoprotein (apo) A-I by human hepatoblastoma cells, Hep-G2. Because of the reported differences in atherogenic cardiovascular mortality between HD and peritoneal dialysis (PD) patients, we examined the effect of similar subfractions from PD patients on apo A-I and apo B synthesis. After obtaining informed consent, serum samples from five normal subjects and nine stable PD patients were applied to Sephadex G-25 columns to obtain the serum subfractions used in the various experiments. Sephadex G-25 chromatograms of PD sera showed a broad peak from fractions 30 through 60 (molecular wt 500 to 2000 Da). Control serum showed no peak in this region. PD serum subfractions decreased apo A-I synthesis, secretion, and apo A-I mRNA expression by Hep-G2 cells when compared to subfractions from control subjects. Cholesterol efflux studies showed that conditioned media obtained from Hep-G2 cells incubated with PD serum subfractions inhibited cholesterol efflux from fibroblasts, suggesting a biologically-significant decrease in apo A-I synthesis. PD serum subfractions increased protein synthesis and mRNA expressions of ...Continue Reading

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Citations

Mar 24, 2010·Nature Reviews. Nephrology·Nosratola D VaziriAlan M Fogelman
Dec 12, 2012·Translational Research : the Journal of Laboratory and Clinical Medicine·Hamid MoradiNosratola D Vaziri
Sep 10, 2010·Journal of Renal Nutrition : the Official Journal of the Council on Renal Nutrition of the National Kidney Foundation·Nosratola D Vaziri
Jan 14, 2009·Translational Research : the Journal of Laboratory and Clinical Medicine·Hamid MoradiNosratola D Vaziri
May 27, 2015·Archives of Medical Research·Valentina KonSergio Fazio
Aug 23, 2017·Expert Opinion on Therapeutic Targets·Marcelino Bermúdez-LópezJose M Valdivielso

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